Research Technician C

Research Technician C

A research technician position is available in the Folkert lab in the Department of Surgery at the University of Pennsylvania. We are seeking a highly motivated individual to work in collaboration with the Principal Investigator, students, residents and/or graduate students to study the immune response to peritoneal surface malignancies, including appendix cancer, colon cancer, and peritoneal mesothelioma. A successful candidate would be detail-oriented, highly organized, and able to work independently after appropriate training and supervision in basic lab techniques and safety. Prior wet lab experience is advantageous, although additional training will be provided. This position will be an excellent opportunity to gain experience performing basic and translational research using patient-derived specimens, offer opportunities for publication, and the chance to work in a collaborative, multidisciplinary setting.

The successful candidate will perform a broad range of routine laboratory tests and procedures to support medical and scientific research. Performs experiments and basic cell biology and immunology techniques, including cell culture, RNA extraction, cDNA synthesis, RT-qPCR, flow cytometry and histology. Collects specimens from the operating room and processes them for downstream assays including cell culture, RNA extraction, sequencing and flow cytometry. Performs mouse experiments including tumor transplants, tumor volume measurements and drug treatments. Assists senior researchers with experiments and provides laboratory support through preparation of media and reagents, equipment upkeep, supply ordering, inventory, and overall lab organization.

Job Responsibilities:

• Conduct experiments and enter findings into computer
• Analyze the results of tests or experiments to ensure conformity to specifications, and generating preliminary figures from data generated towards eventual inclusion in a manuscript
• Processing samples, including collecting samples from the operating room and processing for downstream applications. Preparation and shipping of samples when needed.
• Collect blood or tissue samples from patients.
• Maintaining a detailed log/database of collected specimens.
• Maintain and passage cell cultures
• Optimize novel in vitro culture systems, including slice culture and organoid models
• Perform murine tumor transplants, tumor volume measurements and drug treatments
• Provide scientific and technical guidance for ongoing projects
• Maintain organized laboratory records and ensure compliance with safety protocols
• Maintain inventory of lab supplies, order supplies, oversee general laboratory maintenance and safety compliance
• Collecting and analyzing data through biological assays and microscopy, including cell culture assays, flow cytometry and RT-qPCR
• Perform routine animal studies, including tumor and tissue harvests and preparations as well as drug treatments
• Other duties and responsibilities as assigned

Qualifications:

• Bachelor of Science and 0 to 1 years of experience or equivalent combination of education and experience is required.

This position is contingent upon grant funding.

Job Location - City, State: Philadelphia, Pennsylvania

Department / School: Perelman School of Medicine

Pay Range: $19.23 - $19.23 Hourly Rate

Equal Opportunity Statement: The University of Pennsylvania is an equal opportunity employer. Candidates are considered for employment without regard to race, color, sex, sexual orientation, religion, creed, national origin (including shared ancestry or ethnic characteristics), citizenship status, age, disability, veteran status or any class protected under applicable federal, state or local law.

Special Requirements: Background checks may be required after a conditional job offer is made. Consideration of the background check will be tailored to the requirements of the job.

University Benefits:

• Health, Life, and Flexible Spending Accounts
• Tuition
• Retirement
• Time Away from Work
• Long-Term Care Insurance
• Wellness and Work-life Resources
• Professional and Personal Development
• University Resources
• Discounts and Special Services
• Flexible Work Hours
• Penn Home Ownership Services
• Adoption Assistance