Scientist New Rockville, MD

Axle is a bioscience and information technology company that offers advancements in translational research, biomedical informatics, and data science applications to research centers and healthcare organizations nationally and abroad. With experts in biomedical science, software engineering, and program management, we focus on developing and applying research tools and techniques to empower decision-making and accelerate research discoveries. We work with some of the top research organizations and facilities in the country including multiple institutes at the National Institutes of Health (NIH). Axle is seeking a Scientist to join our vibrant team at the National Institutes of Health (NIH) supporting the National Institute of Neurological Disorders and Stroke (NINDS) located in Bethesda, MD. Benefits We Offer: Paid Time Off and Paid Holidays 401K match up to 5% Educational Benefits for Career Growth Employee Referral Bonus Flexible Spending Accounts: Healthcare (FSA) Parking Reimbursement Account (PRK) Dependent Care Assistant Program (DCAP) Transportation Reimbursement Account (TRN) Position Overview The Scientist will support NINDS's laboratory research program, contributing to the planning and execution of experiments including HIV infection studies in CNS-relevant cell types, plasmid construction and gene-editing work using CRISPR/Cas9, and the evaluation of new laboratory techniques and instrumentation. The role also involves supporting laboratory staff with data interpretation and statistical analysis, maintaining and training others on laboratory equipment, and communicating research findings through technical reports, manuscripts, and presentations at scientific meetings. A Master's degree is required, along with extensive knowledge of virology, cell and molecular biology, molecular genetics, and cellular biochemistry, and a minimum of five years of laboratory experience. Candidates should have strong expertise in molecular biology and genetics techniques, cellular immunophenotype analysis, and cell culture. Certifications & Licenses Extensive knowledge of virology, cell and molecular biology, molecular genetics, and cellular biochemistry, with the ability to analyze and evaluate research findings and learn new techniques. Minimum of five (5) years of laboratory experience. Field of Study Biology Software Microsoft Suite Skills Strong expertise in the techniques of molecular biology and molecular genetics, in cellular immunophenotype analysis, and in cell culture. Meet with lab members to present updates — Ad-Hoc Work products and documents related to cell cultures (cell lines, PBMCs, primary astrocytes, hematopoietic stem cells, iPS, etc.), purification of CD34 cells, stable cell generation, cell migration assay, cell toxicity assay, apoptosis detection, FACS, etc. — Ad-Hoc Work products and documents related to performing HIV isolation and propagation, HIV making by transfection, HIV infection and blocking assays, cocultures and transwell cultures, viral titration, and other HIV-relative experiments. — Ad-Hoc Work products and documents related to performing plasmid construction and preparation, gene expression and detection, gene-editing and HDR by CRISPR/Cas9 technology, and other molecular biology experiments (such as preparation and quantification of DNA and RNA, sequencing, PCR, DNA transfection, RT-PCR, real-time qPCR and so on); perform immunostaining, ELISA, Western blot analysis, cell imaging, phase/fluorescence microscopy, electron microscopy, etc. — Ad-Hoc Work products and documents related to studying HIV, plasmids, primary astrocytes, CRISPR/Cas9 gene-editing. — Ad-Hoc Work products and documents related to interpreting experimental techniques and procedures. — Ad-Hoc Work products and documents related to performing scientific literature searches; write and submit research papers to peer-reviewed scientific journals; contribute to collaborative research projects with other laboratories and departments. — Ad-Hoc Statement of Work Details Perform HIV infection in a variety of CNS-relevant cell types including macrophages and astrocytes to evaluate the effect on expression of HERV-K; determine the relationship between HIV-induced HERV-K expression and development of cellular senescence. — 1 Perform plasmid construction and preparation, gene expression and detection, gene-editing and HDR by CRISPR/Cas9 technology, and other molecular biology experiments (such as preparation and quantification of DNA and RNA, sequencing, PCR, DNA transfection, RT-PCR, real-time qPCR and so on); perform immunostaining, ELISA, Western blot analysis, cell imaging, phase/fluorescence microscopy, electron microscopy, etc. — 2 Evaluate new techniques, instruments, and software for potential incorporation into current SOP. — 3 Work with staff on the interpretation of experiments performed by research scientists. Work with postdoc/biological scientists to perform statistical analysis for research projects. Collaborate on and contribute to research projects with other laboratories and departments. Train staff on the proper usage of laboratory instruments and equipment. Maintain and perform staff training on scientific instrumentation, including HPLCs, microanalytical balances, centrifuges, spectrophotometers, vacuum apparatus, and analytical equipment. Manage scientific equipment and resources (e.g., reagents, stocks of frozen cells, animal samples) including acquisition, documentation, and storage. Participate in the laboratory's review of future proposals for resource allocations. Write technical reports and prepare manuscripts. Present findings at local, national, and international meetings. Work with staff to confirm assay results and evaluate new techniques and instruments; search literature; propose, design, and test alternative assays for difficult screening targets; and develop secondary assays. Salary Range

$110,000 - $113,000 USD

Voluntary Self-Identification As set forth in Axle’s Equal Employment Opportunity policy,we do not discriminate on the basis of any protected group status under any applicable law. If you believe you belong to any of the categories of protected veterans listed below, please indicate by making the appropriate selection.As a government contractor subject to the Vietnam Era Veterans Readjustment Assistance Act (VEVRAA), we request this information in order to measurethe effectiveness of the outreach and positive recruitment efforts we undertake pursuant to VEVRAA. Classification of protected categoriesis as follows: A "disabled veteran" is one of the following: a veteran of the U.S. military, ground, naval or air service who is entitled to compensation (or who but for the receipt of military retired pay would be entitled to compensation) under laws administered by the Secretary of Veterans Affairs; or a person who was discharged or released from active duty because of a service-connected disability. A "recently separated veteran" means any veteran during the three-year period beginning on the date of such veteran's discharge or release from active duty in the U.S. military, ground, naval, or air service. An "active duty wartime or campaign badge veteran" means a veteran who served on active duty in the U.S. military, ground, naval or air service during a war, or in a campaign or expedition for which a campaign badge has been authorized under the laws administered by the Department of Defense. An "Armed forces service medal veteran" means a veteran who, while serving on active duty in the U.S. military, ground, naval or air service, participated in a United States military operation for which an Armed Forces service medal was awarded pursuant to Executive Order 12985. Do you have the legal authorization to work on a full-time, indefinite basis in the country where this role is located? * Select... Can you perform the essential functions of this job without reasonable accommodation? * Select... Are you willing and able to work at the specified job location, or are you open to relocation if required? * Select... Does accepting employment with Axle or performing the duties required for this role conflict with any non‑compete, non‑solicitation, or other contractual agreements you may have? * Select... Do you meet the eligibility requirements for obtaining a US Government Security Clearance? * Select... Do you now, or will you in the future, require sponsorship for employment authorization in the United States? * Select... PUBLIC BURDEN STATEMENT: According to the Paperwork Reduction Act of 1995 no persons are required to respond to a collection of information unless such collection displays a valid OMB control number. This survey should take about 5 minutes to complete. Voluntary Self-Identification of Disability Form CC-305 Page 1 of 1 OMB Control Number 1250-0005 Expires 07/31/2029 Why are you being asked to complete this form? We are a federal contractor or subcontractor. The law requires us to provide equal employment opportunity to qualified people with disabilities. We have a goal of having at least 7% of our workers as people with disabilities. The law says we must measure #J-18808-Ljbffr Axle Info