Research

We are seeking a highly hands-on Research Associate or Scientist to support protein production and quality control workflows as we scale up for our customer's next phase of growth. Working alongside cross-functional scientists and engineers, you will execute, validate, and optimize protein expression, purification, and analytical characterization as part of an automated, high-throughput lab platform. Responsibilities Cell Culture & Expression: Run automated mammalian cell growth and transient transfection for recombinant protein expression, including cell maintenance, expansion, and scaling. Purification: Perform protein purification on AKTA (FPLC) and KingFisher platforms, including affinity capture, polishing, buffer exchange, and magnetic-bead-based processing. Analytical QC: Execute analytical QC by SEC (analytical UPLC/HPLC) to assess purity, aggregation, and monomericity, as well as supporting secondary QC (concentration, endotoxin, SDS‑PAGE). Automation Collaboration: Partner with roboticists and software engineers to translate manual purification and QC steps into reliable, autonomous protocols. Data Analysis & Documentation: Perform first-pass data analysis on chromatograms and SEC traces, maintaining rigorous experimental records in Electronic Lab Notebooks (ELN) and contributing to SOP development. Required Skills B.S. or M.S. in Biochemistry, Molecular Biology, Chemical/Biological Engineering, or a related scientific discipline with hands‑on lab experience. Hands‑on experience personally running or developing 2+ of the following specific assays/instruments: -aSEC purity — Waters UPLC -Hydrophobic interaction (HI) — PAIA Biotech plate assay (Cytation readout) -OVA off‑target binding — Cytation readout -Thermal stability (nanoDSF) — Unchained Labs Aunty -Polydispersity (DLS) — Unchained Labs Aunty Direct hands‑on experience with protein purification (chromatography on AKTA/FPLC and/or bead‑based processing) and taking a sample from lysate/supernatant to purified protein. Strong wet‑lab skills, including mammalian cell culture, aseptic technique, and careful execution of detailed protocols. Basic understanding of antibody structure and function, strong documentation habits, and high attention to detail. Preferred Skills Experience with analytical SEC or other analytical chromatography (UPLC/HPLC). Experience with automated or high‑throughput purification (e.g., KingFisher, multi‑column AKTA, robotic systems). Experience scaling mammalian expression systems (CHO/HEK) and transient transfection. Experience with lab automation or liquid handling platforms (e.g., Dynamic Devices Lynx, Tempest). Familiarity with alternative binder formats (VHHs, bispecifics, Fc‑fusions, or non‑Ig scaffolds). Basic scripting or data‑handling skills (Python/R) for organizing and analyzing purification and QC data. #J-18808-Ljbffr