Molecular Biology Associate - CRISPR Cures Technology Core (9613C) #86345

Position Summary The SRA assists researchers in the CRISPR Cures Technology Core to establish a next generation sequencing protocol to capture both DNA and RNA information from human cells. Application Review Date The first review date for this job is 6/2/2026. Responsibilities PCR from genomic DNA or cDNA to amplify target genes Prepare next generation sequencing libraries to sequence the amplified genes or gene products Extract DNA and RNA from cultured human cells Culture human cell lines Analyze next generation sequencing data to identify mutations and quantify gene expression Required Qualifications Demonstrated proficiency in maintaining mammalian tissue culture cells and performing nucleic acid delivery to them using off-the-shelf technologies such as electroporation and lipid-based delivery. Robust practical familiarity and proficiency with kit-based isolation of DNA and RNA from mammalian tissue culture cells. Robust practical familiarity and proficiency with the end-to-end process of using PCR to amplify specific genomic loci, including primer design and PCR condition optimization, as well as set up of PCR reactions in multiwell format. Demonstrated proficiency in using both agarose gel electrophoresis and PAGE to characterize PCR reaction products. Bachelor's degree in biology or relevant major and/or equivalent experience/training. Preferred Qualifications Library preparation for next generation sequencing Salary & Benefits For information on the comprehensive benefits package, visit the University of California's Compensation & Benefits website. The budgeted hourly range that the University reasonably expects to pay for this position is $28.69 (step 1.0) – $34.22 (step 9.0). This is a non-exempt, bi-weekly paid position. This is a full-time (40 hours/week) career position eligible for full UC benefits. How to Apply Submit your resume and cover letter. Other Information This recruitment has 2 openings. This position is not a visa opportunity and does not include sponsorship of a new consular H-1B visa petition. This position is not eligible for remote work. Misconduct As a condition of employment, the final candidate who accepts a conditional offer of employment will be required to disclose if they have been subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct; received notice of any allegations or are currently the subject of any administrative or disciplinary proceedings involving misconduct; have left a position after receiving notice of allegations or while under investigation in an administrative or disciplinary proceeding involving misconduct; or have filed an appeal of a finding of misconduct with a previous employer. “Misconduct” means any violation of policies or laws governing conduct at the applicant’s previous place of employment, including, but not limited to, violations of policies or laws prohibiting sexual harassment, sexual assault, or other forms of harassment, discrimination, dishonesty, or unethical conduct, as defined by the employer. Equal Employment Opportunity The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law. #J-18808-Ljbffr University of California, Berkeley