Microscopy Scientist Tissue Processing & Expansion Microscopy

Location San Francisco Employment Type Full time Department Structure Compensation $82K – $118K • 0.032% About us: At Eon, we are at the forefront of large-scale neuroscientific connectomic data collection. Our mission is to enable the safe and scalable development of brain emulation technology to empower humanity over the next decade, beginning with the creation of a fully emulated digital twin of a mouse within the next two years. Role Collaborating with a diverse team, including product managers, researchers, and engineering departments, your role involves the processing and staining of brain tissue in preparation for volumetric optical imaging. You will need to contribute to a pipeline that robustly stains large tissue sections and processes them with cutting-edge expansion microscopy techniques. Responsibilities Neural Tissue Staining Optimization : Optimize robust staining of large mammalian neural tissue. Expansion Microscopy Pipeline Development : Help develop an expansion microscopy pipeline producing reproducible outcomes. Cross-Disciplinary Pipeline Collaboration : Work with both animal researchers and macroscopics for upstream and downstream pipeline development. Tissue Processing Method Improvement : Iterate on established tissue processing methods to produce optimized and new methods. Signal Amplification Execution : Execute on signal amplification approaches to realize high signal to noise samples. Antibody Validation : Validate antibodies for successful staining. Volumetric Image Acquisition : Characterize the tissue processing using fluorescence microscopy and work to integrate samples into a microscopy pipeline. Skills and Benefits Experience with screening antibodies for high quality immunofluorescence. Experience with techniques that enable high volume, high-resolution imaging of biological tissues such as Expansion Microscopy, Clarity, or other modern tissue processing methods. General familiarity of neuroanatomy with an eye for proper tissue architecture. Fundamental understanding of fluorescence and fluorophore labeling chemistry. Ability to perform straightforward bioconjugation chemistry. Familiarity of standard fluorescence microscopy techniques as well as advanced version such as confocal and light sheet. Key Projects These are examples of projects that you would be working on when joining us: Developing protocols that result in the timely staining of thick (>1 mm) tissue sample with antibodies and small molecule stains. Synthesis of staining reagents, such as antibodies, that possess a high intensity of fluorescent signal. Processing of an entire mammalian brains via serial sections for microscopic analysis. Performing Expansion Microscopy regularly on tissue sample and pushing the state of the art in the field. Salary and Benefits Competitive salary and equity. Compensation Range: $82K - $118K #J-18808-Ljbffr