RESEARCH TECHNICIAN III

School of Medicine Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,500 faculty physicians and researchers, more than 1,300 students, and more than 6,000 staff, the Duke University School of Medicine along with the Duke University School of Nursing, Duke University Health System and the Private Diagnostic Clinic (PDC) comprise Duke Health. a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Primary Care, Duke Home and Hospice, Duke Health and Wellness, and multiple affiliations.

Be You.

Department of Radiation Oncology
Collaborating with the Preston Robert Tisch Brain Tumor Center, Department of Neurosurgery, and Duke Cancer Institute

About the Lab

The Sulman Lab investigates fundamental mechanisms driving cancer development and progression, with the ultimate goal of identifying better treatments for patients. We employ advanced molecular biology approaches, functional genomic screening, single-cell technologies, and our in-house CARPOOL platform for high-throughput drug evaluation.

Research Focus

• Treatment resistance and radiation response
• Genetic and pharmacologic screens in cellular and organoid models
• Functional genomics and 3D chromatin profiling to study enhancer function
• Tissue processing and molecular analysis of patient-derived samples

Qualifications

• Undergraduate degree completed by start of employment
• Prior laboratory research experience
• Intellectually passionate and able to display scientific initiative
• Candidates will gain the most from the experience if they are able to stay for more than 1 year of time, ideally 2 years or more

Be Bold.
Responsibilities

• Maintain an organized and complete lab notebook of experiments
• Propagate cells in culture; perform viability and clonogenic assays
• Establish and maintain cancer organoid cultures from human specimens
• Perform PCR, RTPCR, and Western blots on cell lines, tumor tissues, and mouse tissues
• Learn and implement advanced techniques: highthroughput microscopy, confocal microscopy, flow cytometry
• Learn and implement nextgeneration sequencing library preparation (analysis available for motivated candidates)
• Assist with mouse model experiments as needed
• Meet regularly with supervisor to plan experiments, troubleshoot, and discuss results
• Participate in lab meetings to present results or discuss literature
• Assist in recruiting new lab members and orienting them to lab research
• Help maintain reagents and equipment (e.g., buffers, ethanol)
• Contribute to a collegial laboratory environment
• Set up instruments, materials, and apparatus; operate standard laboratory equipment
• Participate in laboratory logistics: inspections, ordering, equipment repair

Application

Interested candidates should send a CV and cover letter to View email address on click.appcast.io and also apply via this posting.

Choose Duke.

Anticipated Pay Range:

Duke University provides an annual base salary range for this position as USD $22.61 to USD $34.27. Duke University considers factors such as (but not limited to) scope and responsibilities of the position; candidate's work experience, education/training, and key skills; internal peer equity; as well as market and organizationalconsiderations when extending an offer.

Your total compensation goes beyond the dollars on your paycheck. Duke provides comprehensive and competitive medical and dental care programs, generous retirement benefits, and a wide array offamily- friendly and cultural programs to eligible team members. Learn more at:

Education

Work generally requires skills acquired through the completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.

Experience

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Degrees, Licensures, Certifications

Work requires two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience. OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE

Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends on the robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions:

Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.