Research Technician II - Pediatrics Genetics

Scheduled Hours: 40 Position Summary The Greer lab is interested in how non‑genetic information can be transmitted across generations and how non‑genetic information regulates complex phenotypes. An increasing number of complex phenotypes, such as physical appearance, energy metabolism, psychological state, and longevity, have recently been shown to be regulated, in part, by epigenetic information. Epigenetics describes how gene expression changes occur without changes to the DNA sequence. Proteins, RNA molecules, or chemical modifications to histones or DNA can induce these epigenetic changes. How this information, which is not directly coded in our DNA, is passed from generation to generation is still unknown. Understanding the molecular determinants of stable epigenetic memory will provide insight into how environmental changes can affect the health and lifespan of not only the individual who experiences them, but also of their progeny. Our goals are to identify epigenetic inheritance phenotypes and to elucidate the mechanisms behind their transmission across generations. We also aim to understand how epigenetic mis‑regulation can cause developmental defects and disease. In the Greer lab we use the nematode C. elegans , the social amoeba Dictyostelium, mice, and mammalian tissue culture systems to study how non‑genetic information can be transmitted across generations as well as to understand the role of non‑genetic information in regulating a variety of biological processes. We use a wide variety of genetic, biochemical, and molecular techniques to decipher these complex questions. Some experiments would entail worm, Dictyostelium, or mouse husbandry, extracting gDNA and proteins from worm and tissue samples, running dot blots and western blots, purifying proteins and testing their enzymatic activity. Assists with the technical aspects of studies and experiments, including documentation and preparation of materials. The Greer lab strives to be a place of fun, collegiality, and collaboration. Prior research experience with any of the following: tissue culture, C. elegans , Dictyostelium discoideum, or mice, would be preferred for this position. Primary Duties & Responsibilities Assists with research studies, experiments and assays, including collection of data, preparation of solutions and setup and maintenance of equipment. Performs data entry and maintains data files on research. Prepares tissue cultures, reagents, probe tests, etc. Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions. Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures. Assists with general lab maintenance and cleaning. Performs other duties as assigned. Working Conditions This position works in a laboratory environment with potential exposure to biological and chemical hazards. The individual must be physically able to wear protective equipment and to provide standard care to research animals. The above statements are intended to describe the general nature and level of work performed by people assigned to this classification. They are not intended to be construed as an exhaustive list of all job duties performed by the personnel so classified. Management reserves the right to revise or amend duties at any time. Required Qualifications Education: Bachelor’s degree or combination of education and/or experience may substitute for minimum education. No specific work experience is required for this position. No specific certifications or professional licenses are required. The ideal candidate will have up to one year of experience in a laboratory setting. Communication, detailed reports, laboratory instrumentation, laboratory research, software program, and ability to work independently. Salary and Classification Grade: R06-H Salary Range: $16.92 - $26.44 per hour. EEO Statement Washington University in St. Louis is committed to the principles and practices of equal employment opportunity. It is the University’s policy to provide equal opportunity and access to persons in all job titles without regard to race, ethnicity, color, national origin, citizenship, age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. #J-18808-Ljbffr Washington University in St. Louis