Research Technician in Cell and Molecular Biology

Research Technician Position

A Research Technician position in Cell and Molecular Biology is available at the Genomic Research Center (GRC) at Client. We are seeking a highly motivated research technician to work independently and collaboratively with other scientists on projects involving human-induced pluripotent stem cell (hiPSC) reprogramming, cell differentiation, CRISPR editing, high-throughput functional screening, and a variety of molecular biology experiments in the wet lab. The successful candidate will be responsible for performing time-sensitive experiments and supporting extended hiPSC differentiation protocols, both of which require daily attention to detail.

Responsibilities:

• Conduct routine experimental protocols and procedures in the wet lab.
• Maintain tissue culture, including stem cells and stem cell-derived cells.
• Accurately record experimental setups, SOP protocols, and data quality control.
• Maintain efficient and continuous communication with the manager and team members.
• Support team members with routine cell culture procedures, including media preparation and reagent aliquoting.
• Conduct molecular and cell biology experiments such as qRT-PCR, immunofluorescence, and Western blot analyses.

Requirements:

• A bachelor's or master's degree in biochemistry or molecular cell biology, with at least three years of independent hands-on laboratory experience.
• Trained and experienced in using aseptic techniques in a laminar flow biosafety cabinet.
• Significant cell culture expertise, including maintenance, freezing, thawing, and passaging.
• Proficiency in biochemical assays such as Western blot or WES, immunofluorescence, and flow cytometry.
• Skilled in molecular biology techniques such as RNA extraction, cDNA synthesis, and RT-qPCR.
• Knowledge of serial dilution and calculation of solution concentration.
• Excellent written and oral communication skills.
• Organized in planning long experimental protocols that may sometimes require working on weekends.
• Nice to have: Experience with culturing human iPSC and differentiating iPSC into brain cell types.