Scientist / Senior Scientist, In Vivo CAR-T

Addition Therapeutics is at the forefront of biotechnology innovation, advancing transformative therapies utilizing its proprietary PRINT™ (Precise RNA-mediated Insertion of Transgenes) platform. PRINT™ redefines genetic medicine by enabling development of RNA-only therapeutics capable of inserting any genetic element into specific safe harbor sites within the genome. Operating from state-of-the-art labs in South San Francisco, our team of experienced scientists and drug developers is leading scientific breakthroughs while cultivating a vibrant, dynamic culture committed to profoundly impacting patient lives.

Job description:

Addition Therapeutics is seeking a highly skilled and motivated Scientist / Senior Scientist to join the Preclinical Translation team and lead the design and hands-on execution of in vivo CAR-T studies. This role will have a strong emphasis on experimental execution, animal handling, model development, and data interpretation.

The ideal candidate will have experience with humanized mouse and tumor models for CAR-T, in vivo assessment of tumor killing, and characterization of CAR-T expansion, persistence, and activity. The individual will work closely with scientists across Preclinical, Disease Biology, CMC, and Discovery functions to generate and integrate in vivo efficacy and cellular / molecular readouts to advance CAR-T programs.

The successful candidate will bring technical expertise, operational excellence, and strong leadership to ensure high-quality, reproducible, and compliant in vivo data generation. They will be a highly collaborative and resourceful scientist with superior communication skills who can work effectively in a fast-paced, matrixed setting. This role has vast learning opportunities in the field of genomic medicines and presents a unique opportunity to explore the multifaceted applications of PRINT™.

Key Responsibilities:

• Develop and optimize in vivo models to evaluate CAR-T efficacy, tumor killing, expansion, persistence, and activity.
• Design and execute in vivo CAR-T studies in appropriate humanized mouse models.
• Perform animal handling and procedures, including dosing, blood collection, tissue and tumor harvest, and sample processing for downstream analyses.
• Perform and interpret multiparameter flow cytometry analyses to characterize CAR-T cells and immune populations.
• Support molecular and protein-based analyses, as needed
• Integrate in vivo efficacy, cellular, and molecular readouts to guide program decisions.
• Develop study protocols and contribute to IACUC amendments, animal welfare, compliance, and study documentation.
• Manage external studies with CROs, including vendor selection, protocol design, oversight, and data review, analysis, and interpretation.
• Troubleshoot experimental challenges and drive improvements in study execution and model performance.
• Maintain detailed and organized study records; contribute to study reports and presentations.

Qualifications

• PhD in Biology, Pharmacology, Immunology, Cancer Biology, or related field, with 4-6 years of relevant research or industry experience.
• Demonstrated hands-on in vivo pharmacology experience, including animal handling, dosing, tissue collection, and necropsy.
• Experience with CAR-T in vivo models, preferably humanized mice and tumor models.
• Experience assessing in vivo tumor killing/efficacy and CAR-T expansion or persistence.
• Proficiency with flow cytometry and data analysis.
• Experience managing in vivo studies at CROs
• Experience with ddPCR, ELISA/MSD, or related molecular/protein assays is preferred.
• Strong experimental, analytical, troubleshooting, organizational, and communication skills.
• Meticulous attention to detail and strong documentation including use of electronic notebook for record keeping.
• Team player who fosters a culture of collaboration, accountability, and scientific excellence, and who thrives in a fast-moving, results-oriented startup environment.
• A self-motivated, driven individual with a passion to learn and can-do attitude.

Compensation : The expected yearly salary for this position is $116,000-$166,000. Exact compensation may vary based on skills and experience.

Location: South San Francisco, California

We are an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status.