Protein Expression Scientist

Position Summary

The Lead Discovery and Structural Biology Department is seeking a highly qualified Protein Expression & Purification Scientist with demonstrated expertise in molecular biology, heterologous protein expression, and protein purification. The successful candidate will contribute to research programs within an industry-leading drug discovery environment leveraging access to state-of-the-art protein expression and purification platforms. Responsibilities include executing expression workflows in baculovirus expression vector systems (BEVS)/insect cells, as well as in E. coli, including high-throughput (HTP) small-scale expression screening, followed by downstream purification and analytical characterization. Experience with biophysical methods is considered an asset.

Essential Duties and Responsibilities

• Perform molecular biology workflows, including:
• Cloning, vector design, construct optimization, and sequence verification
• PCR, primer design, restriction based, and recombination-based cloning
• Execute protein expression using:
• BEVS/insect cell systems, including virus generation, amplification, and expression optimization
• E. coli expression, including IPTG induced and auto-induction strategies
• High throughput (HTP) small scale expression testing for rapid screening of constructs and conditions
• Conduct purification of recombinant proteins using standard chromatographic techniques (affinity, ion exchange, size exclusion chromatography).
• Optimize expression constructs, culture conditions, and purification workflows to achieve the required yield, purity, and quality.
• Perform analytical characterization, including SDS PAGE, Western blotting, SEC, and other relevant assays.
• Maintain accurate and complete laboratory records in accordance with internal documentation standards (experience with Benchling is a plus).
• Communicate experimental results, observations, and recommendations to team members in a clear and timely manner.
• Adhere to all laboratory safety policies and organizational compliance requirements.

Education and Experience

• Bachelor’s degree in Biochemistry, Molecular Biology, Structural Biology, or related field with 3+ years of relevant industry experience, or
• Master’s degree with 1+ year of relevant industry experience
• Strong molecular biology and cloning expertise is required.
• Proven track record of protein expression in BEVS/insect systems and E. coli, including HTP small scale expression screening.
• Demonstrated proficiency in chromatographic purification and method development.
• Experience with biophysical characterization techniques (e.g., LC?MS, SPR, MST, SEC?MALS, nanoDSF, mass photometry) is preferred but not required.

Knowledge, Skills, and Abilities

• Strong analytical and troubleshooting skills across cloning, expression, and purification workflows.
• Ability to work independently while contributing and collaborating effectively with cross-functional teams.
• Excellent organizational, documentation, and communication skills.
• Ability to manage multiple tasks and meet project timelines in a fast-paced environment.