Research Associate III

Execute and troubleshoot production and QC of neuronal cultures for Quiver’s multiple customer-based and internal R&D projects. Maintain and characterize multiple human iPS cell lines. Use transcriptional programming and directed differentiation strategies to produce various types of high-quality human iPS cell-derived neurons, including excitatory, inhibitory, sensory and motor neurons. Plate and maintain cultures of human iPS cell-derived neurons in 96-well and 384-well assay formats. Carry out RNA and DNA preparations from human cell lines and cultured neurons. Perform candidate gene expression analysis using quantitative PCR assays. Assess protein expression using immunocytochemistry (ICC) and immunoblotting assays. Assist with cell culture lab operations, including inventory, purchasing of lab reagents and supplies, and aliquoting of recombinant protein and lentiviral particle stocks. Communicate work progress via technical reports, PowerPoint presentations, and seminars at internal meetings. Responsibilities And Duties Execute and troubleshoot production and QC of neuronal cultures for Quiver’s multiple customer-based and internal R&D projects. Maintain and characterize multiple human iPS cell lines. Use transcriptional programming and directed differentiation strategies to produce various types of high-quality human iPS cell-derived neurons, including excitatory, inhibitory, sensory and motor neurons. Plate and maintain cultures of human iPS cell-derived neurons in 96-well and 384-well assay formats. Carry out RNA and DNA preparations from human cell lines and cultured neurons. Perform candidate gene expression analysis using quantitative PCR assays. Assess protein expression using immunocytochemistry (ICC) and immunoblotting assays. Assist with cell culture lab operations, including inventory, purchasing of lab reagents and supplies, and aliquoting of recombinant protein and lentiviral particle stocks. Communicate work progress via technical reports, PowerPoint presentations, and seminars at internal meetings. Minimum Qualifications Required A Master’s degree in biology, cell biology, biotechnology or a related field. At least two years of experience with mammalian cell culture (in particular iPS cell-derived neurons) and with DNA/RNA/Protein preparations and readout assays (PCR, qPCR, immunoblotting, and ICC). Ability to work independently and in cross-disciplinary teams. Excellent oral, written and interpersonal communication skills. Additional Qualifications Desired Experience with methods for human iPS cell differentiation into neurons is a plus. Experience with 96/384-well cultures and high-throughput screening is a plus. Experience with neuronal preparations from rodent CNS tissues is a plus. #J-18808-Ljbffr