Research Technician II - Pediatrics Newborn Medicine

Position Summary The newly forming Alfano lab focuses on the mechanisms of pathogen-specific molecular determinants of sepsis and endothelial dysfunction. The goal of our research is to determine the factors that confer increased risk of life‑threatening sepsis to certain populations and provide novel insights in future therapeutic developments. We utilize extensive in vivo and in vitro modeling of bacterial infection in concert with both bacterial and host genetic perturbation to examine the host‑bacterial interaction. Experimental approaches involve endothelial cell culture, bacterial culturing, microscopy, flow cytometry, molecular biology, functional cell‑based in vitro assays, and in vivo murine models. Scheduled Hours: 40 hours per week. The Research Technician will work closely with the PI and other members of the laboratory in studies examining mouse models of infection and endothelial dysfunction. Assists with the technical aspects of studies and experiments including documentation, preparation of materials, and assisting with analysis of results. Our work operates alongside the mentorship of the Bubeck Wardenburg lab at WashU Medicine. This position offers the opportunity to join an innovative research team and contribute to basic and translational research projects. Primary Duties & Responsibilities Assists with research studies in both in vitro and in vivo systems, experiments, and assays including collection of data, preparation of reagents, set‑up and maintenance of equipment. Assists with the maintenance of the laboratory’s mouse colony (e.g., breeding and weaning mice, and genotyping for maintenance of genetic integrity of the colony). Performs data entry, maintains data files on research, and assists with analysis. Complies with established safety procedures and maintains required documentation on laboratory and specimen conditions. Ensures lab conditions and equipment are properly cleaned and maintained in accordance with established procedures. Assists with general lab maintenance including ordering and shipping. Working Conditions This position works in a laboratory environment with potential exposure to biological, chemical, and BSL2 pathogens. The individual must be physically able to wear protective equipment and to provide standard care to research animals. Education & Qualifications Required Qualifications: Bachelor’s degree or combination of education and/or experience may substitute for minimum education. Certifications / Professional Licenses No specific certification or professional license is required for this position. Work Experience No specific work experience is required for this position. Skills Not applicable. Driver’s License A driver’s license is not required for this position. More About This Job The ideal candidate will have up to one year of experience in a laboratory setting. Preferred Qualifications No additional education unless stated elsewhere in the job posting. Work Experience: Laboratory setting (1 year). Relevant skills: Blotting techniques, cell cultures, cellular biology techniques, cloning, communication, detailed reports, flow cytometry, laboratory instrumentation, laboratory research, microscopy techniques, molecular techniques, mouse handling, polymerase chain reaction (PCR), software program, working independently. Grade & Salary Grade: R06-H Salary Range: $16.92 - $26.44 per hour. Benefits Statement Personal Benefits Up to 22 days of vacation, 10 recognized holidays, and sick time. Competitive health insurance packages with priority appointments and lower copays/coinsurance. Take advantage of our free Metro transit U-Pass for eligible employees. WashU provides eligible employees with a defined contribution (403(b)) retirement savings plan, which combines employee contributions and university contributions starting at 7%. Wellness Benefits Wellness challenges, annual health screenings, mental health resources, mindfulness programs and courses, employee assistance program (EAP), financial resources, access to dietitians, and more! Family Benefits We offer 4 weeks of caregiver leave to bond with your new child. Family care resources are also available for your continued childcare needs. Need adult care? We’ve got you covered. WashU covers the cost of tuition for you and your family, including dependent undergraduate-level college tuition up to 100% at WashU and 40% elsewhere after seven years with us. For policies, detailed benefits, and eligibility, please visit: EEO Statement Washington University in St. Louis is committed to the principles and practices of equal employment opportunity. It is the University’s policy to provide equal opportunity and access to persons in all job titles without regard to race, ethnicity, color, national origin, citizenship (where prohibited by federal law), age, religion, sex, sexual orientation, gender identity or expression, disability, protected veteran status, or genetic information. #J-18808-Ljbffr Washington University in St. Louis