SENIOR BACTERIOLOGY TECHNICIAN B

Cape Fox subsidiary Eagle Health, LLC is accepting resumes for a SENIOR BACTERIOLOGY TECHNICIAN to support the members at Ft. Detrick, Maryland. This position is contingent upon contract award. Incumbents are encouraged to apply! The salary range for this exempt position has been established at: $85,000-$95,000 . This job offers a variety of benefits including company holidays, paid time off, health insurance, dental insurance, vision insurance, life and disability insurance, tuition reimbursement, and 401K with company match. This job will be posted until filled or withdrawn. Responsibilities Assist the Lead/PI in molecular microbiological experiments to define elements and factors involved in bacterial virulence; assist with approximately two animal protocols conducted by other investigators. Complete approximately 25 opsonic adherence assays to test the efficacy of animal sera (rabbit and NHP) in the presence of baby rabbit complement to adhere Ames bacilli and spores to RAW 264.7 macrophage cells. Complete over 1000 ELISAs, including retesting of samples where needed: Yersinia pestis novel vaccine strategies Burkholderia novel vaccine strategies Yersinia pestis novel mAb identification Burkholderia novel mAb identification Francisella tularensis novel vaccine strategies/animal modeling Coxiella burnetii novel vaccine strategies/animal modeling Prepare approximately 10 different reagents/media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc). Plate approximately 100 LB plates for Ames sporulation isolation and purification. Isolate and purify approximately 10 plasmid DNA specimens. FITC label and count approximately 10 Ames samples to check the ratio of bacilli to spores over multiple time points. Order lab supplies for BSL-2 and BSL-3 laboratories, including common supplies, custom orders, and equipment—approximately 400 items ordered along with 5 pieces of equipment. Present data from opsonic adherence and ELISA at group meetings; prepare graphs and reports for the supervising investigator. Perform approximately 100 immunological antibody determinations. Perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum. Perform approximately 50 cytokine analyses using a Luminex instrument. Perform approximately 50 flow cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression, using a BD FACS Calibur and BD FACS Canto II. Perform approximately 25 cell biology/biochemical/microbiological tasks, including laboratory tests such as protein gel electrophoresis, western blot analysis, growth of macrophage cell lines, live-dead cell determinations, and media preparation for bacterial cultures. Organize and maintain data from immunological and microbiological studies. Manage approximately 80 hand receipt items and maintain laboratory equipment. Complete approximately 10 anthrax toxin neutralization assays per year. Perform approximately 15 animal challenges per year and approximately 10 bacterial challenge preparations. Perform approximately 1,200 animal injections per year and approximately 2,500 animal observations per year. Complete approximately 500 bacterial burdens in organ tissue per year. Perform approximately 100 Luminex cytokine analyses per year. Perform approximately 75 macrophage phagocytosis assays per year. Process approximately 200 ELISAs per year. Perform approximately 125 animal necropsies per year. Process approximately 150 animal gavage and 250 animal blood collections per year. Complete approximately 40 DNA chromosomal preparations, 500 PCR reactions, 35 agar gel analyses per year. Prepare approximately 20 cloning plasmids, 50 sequencing reactions, and 90 plasmid preparations. Complete approximately 100 media preparations per year. Perform approximately 250 BSL-3 live/dead assays per year. Process approximately 40 protein and western gels per year. Place approximately 50 GFEBs orders per year for supplies and equipment. Review relevant literature monthly. Serve as a training representative for BSL-2 laboratory skills. Assist the PI with project planning quarterly. Participate in training new personnel on laboratory tasks monthly. Perform BSAT inventory management weekly. Conduct equipment inventory management twice a year in BSL-2 and BSL-3. Perform monthly laboratory management, including safety functions and chemical inventory. Assist the BSL-3 suite supervisor with suite supervisory duties. Interface with inspectors and perform monthly inspections. Plan and perform animal experiments under BSL-3 conditions. Review relevant literature monthly; assist in preparing standard operating procedures and manuscripts; present research findings at one scientific conference per year and present results in written and oral format. Provide one bacteriology technical support monthly report to TPOC by the last working day of the month. CS-PRP enrollment is required and shall be maintained IAW the provisions in this contract and all applicable regulations. BPRP enrollment is required and shall be maintained IAW the provisions in this contract and all applicable regulations. Cape Fox Corporation, its subsidiaries, and affiliates provide equal employment opportunities to all persons and prohibit employment decisions on the basis of race, religion, color, creed, national origin, sex, age, disability, political affiliation, protective veteran status, or sexual orientation, or any other characteristic protected by federal, state, or local law. Cape Fox Corporation, its subsidiaries, and affiliates offer preference to Cape Fox Corporation shareholders, descendants, and other Native Americans pursuant to Public Law 100-241. #J-18808-Ljbffr Cape Fox Shared Services