Research Technologist 2, Cardiology

Job Summary Conducts experimental research lab work and/or assists in design of one or more specific phase(s) of entire project(s). May complete mathematical calculations, computer tasks and assist in judging validity of data. Follows appropriate protocols, policies, procedures, regulations, Good Clinical and Good Laboratory Practices. Specific Responsibilities Following protocols, designs, sets-up and conducts basic experiments. Analyzes and troubleshoots protocols and data obtained from individual experiments and recommends alternatives and suggestions to improve processes, validity and/or accuracy. Using a fully functioning level of skill, completes the following activities: Molecular Biology DNA, RNA, Protein isolation, purification and expression; Southern, Northern, Western blotting

PCR, RT-PCR

Plasmid preparation Cloning, mapping and sequencing of DNA Recombinant virus production and purification Cellular Biology Cell culture and cell isolation Functional studies Transfection Histology Tissue fixation/processing: embedding, preparation of blocks and sectioning, staining Immunohistochemistry Microscopy Immunology Flow cytometry ELISA, Elispot assay Cytokine assay Antibody production and purification Animal Husbandry Breeding and screening transgenic mice Dissections Blood collection Surgical procedures Database management of colony Administration Maintains detailed records of results, including collecting, extracting & entering data. Manipulates, interprets &or presents results. Prepares charts & graphs for presentations & publications. Performs scientific literature searches in support of research. Completes portions of grant applications, manuscripts & publications. Finance Tracks & summarizes lab supply expenses. Verifies accuracy of invoices. Orders & maintains inventory of reagent, mice & lab supplies. Supervises Trains students. Minimum Qualifications Successful completion of a full 4‑year course of study in an accredited college or university leading to a bachelor's or higher degree in a scientific major or related; OR appropriate combination of education and experience. Minimum Competencies Demonstrated laboratory experience; Demonstrated ability to follow detailed instructions as required by research protocols; Demonstrated computer skills, including spreadsheets and basic computations; Attention to detail and accurate record keeping; Ability to work independently and in a team environment; Manual dexterity. Preferred Qualifications Two years of practical laboratory (clinical or research) working experience in molecular biology with a focus on nucleic acid research. Prior working experience involving small RNA isolation, extraction, library preparation, and sequencing. Preferred Competencies Skill/knowledge in common genomic techniques including high throughput assays such as microarrays, qPCR, and sequencing. Action oriented – Willing or likely to take practical action to deal with a problem or situation. Coachability – Receptive to feedback, willing to learn, embracing continuous improvement. Collaboration – Facilitates open and effective communication, cooperation and teamwork within and outside of one's own team. Collegiality – Helpful, respectful, approachable; builds strong working relationships and a positive work environment. Initiative – Exhibits energy and desire to achieve; sets ambitious goals and acts decisively; takes action that no one has requested to improve or enhance job results and avoid problems. Meets deadlines – Displays consistency and success in adhering to deadlines. Multi‑tasking – Demonstrates ability to work on multiple projects simultaneously. Organization – Plans, organizes and schedules in an efficient, productive manner; anticipates contingencies and pays attention to detail. Salary Target hiring range for this position will be between $19.89 – 27.97 per hour. Offered salary will be determined by the applicant's education, experience, knowledge, skills and abilities, as well as internal equity and alignment with market data. Benefits At Northwestern, we are proud to provide meaningful and competitive benefits. The available benefits package for this position will include health, dental, vision, disability, and life insurance; paid vacation and holidays; paid medical/sick and parental leave; tuition benefits for the employee and dependents; pre‑tax and flex spending accounts for commuting and dependent care; generous retirement savings options; and wellness programs. For a comprehensive overview of available benefits, including eligibility details, visit us at to learn more. Work‑Life and Wellness Northwestern offers comprehensive programs and services to help you and your family navigate life's challenges and opportunities and adopt and maintain healthy lifestyles. We support flexible work arrangements where possible and programs to help you locate and pay for quality, affordable childcare and senior/adult care. Visit us at to learn more. Professional Growth & Development Northwestern supports employee career development in all circumstances whether your workspace is on campus or at home. If you're interested in developing your professional potential or continuing your formal education, we offer a variety of tools and resources. Visit us at to learn more. Equal Opportunity Employment Northwestern University is an Equal Opportunity Employer and does not discriminate on the basis of protected characteristics, including disability and veteran status. Job applicants who wish to request an accommodation in the application or hiring process should contact the Office of Civil Rights and Title IX Compliance. View additional information on the accommodations process. #J-18808-Ljbffr Northwestern University