Technical Assistant I/II - Cheeseman Lab (Relocation Assistance Available!)

Cheeseman Lab Research Technician

About The Cheeseman Lab - Analyzing Cell Biology at Scale

The Cheeseman lab seeks an enthusiastic and motivated individual with boundless curiosity who is interested in conducting research to probe the inner workings of human cells. The work in the Cheeseman lab is focused on understanding core cell biological questions, including applying fluorescence microscopy and functional genetic strategies to define the requirements for cell division and other cellular processes. Over the past few years, we have conducted pooled cell biological profiling using Cas9-based gene targeting for thousands of genes in human cells, acquiring microscopy-based images for close to a hundred million cells. We have also been at the forefront of both computational and automation advances for optical pooled screens to improve success and throughput. We are recruiting a proactive researcher to contribute to these large-scale cell biology efforts to extend this approach to diverse cell biological markers, cell lines, and different cell states unlocking fundamental new biological discoveries. This collaborative project and our dynamic environment provide strong opportunities for training, growth, and professional development.

Overall Responsibility

End to end ownership of a variety of optical pooled screens to drive scientific research objectives of the lab. Involves cell culture maintenance, high-throughput microscopy instrumentation, and the use of dedicated fluidics automation to rapidly generate screens. Addresses methodological problems in experimental protocols and results, reports data to supervisor, and presents results. May include analysis of optical pooled screening data. Uses discretion in prioritizing and organizing workflow of large-scale screening efforts and in optimizing methodologies and results. Contributes to lab organization and operation.

Characteristic Duties

• In consultation with the Principal Investigator and the broader optical pooled screening team, contributes to the design and execution of optical pooled screens.
• In coordination with the wet-lab pooled screening team, contributes to marker optimization and cell culture operations.
• Conducts optical pooled screens at scale using custom microfluidics automation devices for reagent exchange. May contribute to further development of automation setup.
• With assistance from the computational pooled screening team, learns to execute analyses of pooled screening data autonomously.
• Documents, compiles, and assists in interpreting experimental data. Reports data to supervisor in oral and written reports.
• Gives presentations on research findings, including lab meetings and institute-wide meetings.
• Consults the scientific literature as needed.
• Other tasks as required or assigned.

Qualifications

• Bachelor's degree in biology or a field directly related to the work.
• Must have 2+ years of experience working in a research laboratory. *2 years of FT lab experience is required for the Technical Assistant II title.
• A background in molecular biology or cell biology is desired, including microscopy-based approaches.
• Proficient in basic lab techniques and principles of molecular biology required.
• Experience with computational analysis, use of agentic coding platforms, and some knowledge of high-performance computing platforms is a plus.
• Knowledge of various scientific databases and publications preferred.
• Excellent written and oral communication skills, strong organizational skills required.

Technical Assistant I Pay: 47,500

Technical Assistant II Pay Range: 48,000 56,000

Whitehead Institute for Biomedical Research