Scientist - Protein Biochemist

Scientist Protein Biochemist Join Amgen’s mission to serve patients. In this vital role you will be responsible for designing, planning, and executing the generation of key enabling reagents that support the structure determination of target molecules via X‑ray crystallography and/or cryo‑electron microscopy (cryo‑EM). You will collaborate closely with structural biologists and contribute to advancing our drug discovery efforts. Responsibilities Participate in design and execution of molecular cloning and protein expression strategies with the Amgen Discovery Protein Science team or CROs; design and execute purification strategies to support the structure determination of drug targets. Work closely with structural biologists to generate high‑quality protein samples for structural biology techniques, especially X‑ray crystallography and cryo‑EM. Develop and optimize purification protocols and reagents to ensure high protein purity, homogeneity, and stability for structural studies. Use biochemical and biophysical techniques (e.g., SDS‑PAGE, Western blot, spectrophotometry, SEC‑MALS, SPR, mass spectrometry) to assess protein quality and stability, and collaborate with team members to provide mechanistic insights into the mechanism of action of proteins involved. Manage multiple projects simultaneously, ensuring they are completed within timelines while meeting high‑quality standards. Present research findings effectively in written and oral formats for internal teams and external collaborators. Continuously troubleshoot and optimize experimental workflows, applying creative solutions to overcome challenges in protein production and purification. Basic Qualifications Doctorate degree, or Master’s degree and 2 years of molecular or structural biology experience, or Bachelor’s degree and 4 years of molecular or structural biology experience. Preferred Qualifications Experience with microbial systems (e.g., E. coli or yeast) or cell culture (mammalian or insect cells) for protein expression. Hands‑on experience with biochemical analytical techniques such as spectrophotometry, SDS‑PAGE, Western blot, SEC‑MALS, SPR, and basic mass spectrometry. Familiarity with chromatography systems (e.g., Cytiva AKTA, Agilent HPLC) and related software. Expertise in protein purification methods, including affinity, ion exchange, hydrophobic interaction (HIC), and size‑exclusion chromatography (SEC). Experience with biophysical characterization of protein complexes, including DLS and analytical ultracentrifugation (AUC). Experience with expression and purification of membrane proteins. Strong knowledge of protein stability, folding, and quality control for structural studies. Excellent presentation skills (written and oral) to communicate research results to scientific and cross‑functional teams. Ability to work independently, manage multiple projects, and meet deadlines in a collaborative environment. Demonstrated ability to prioritize tasks and manage projects efficiently. Benefits Amgen offers a comprehensive total rewards plan, including health and welfare plans for staff and eligible dependents; financial plans with retirement savings opportunities; flexible work models where possible; discretionary annual bonus program; stock‑based long‑term incentives; award‑winning time‑off plans; and career development opportunities. Amgen is an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability status, or any other basis protected by applicable law. We will ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation. #J-18808-Ljbffr BioSpace