Scientist - Protein Biochemist

Scientist – Protein Biochemist In this vital role you will be responsible for designing, planning, and executing the generation of key enabling reagents that support the structure determination of target molecules via X‑ray crystallography and/or cryo‑electron microscopy (cryo‑EM). You will collaborate closely with structural biologists within the group and contribute to advancing our drug discovery efforts. The ideal candidate must possess flexibility in applying their knowledge to new areas of study and have strong interpersonal and communication skills to thrive in a collaborative environment. Responsibilities Be actively involved in the design and execution of molecular cloning and protein expression strategies with the Amgen Discovery Protein Science team or CROs; design and execute purification strategies to support the structure determination of drug targets. Work closely with structural biologists to generate high‑quality protein samples for structural biology techniques, particularly X‑ray crystallography and cryo‑EM. Develop and optimize purification protocols and reagents, ensuring high protein purity, homogeneity, and stability for structural studies. Use biochemical and biophysical techniques (e.g., SDS‑PAGE, Western blot, spectrophotometry, SEC‑MALS, SPR, mass spectrometry) to assess protein quality and stability. Collaborate with team members to provide mechanistic insights into the mechanism of action (MOA) of proteins involved in the associated proteins. Manage multiple projects simultaneously, ensuring they are completed within timelines while meeting high‑quality standards. Present research findings effectively in both written and oral formats for internal teams and external collaborators. Continuously troubleshoot and optimize experimental workflows, applying creative solutions to overcome challenges in protein production and purification. What We Expect Of You The Scientist we seek is a highly motivated professional with the following qualifications. Basic Qualifications Doctorate degree Master’s degree and 2 years of Molecular, Structural Biology experience Bachelor’s degree and 4 years of Molecular, Structural Biology experience Preferred Qualifications Experience with microbial systems (e.g., E. coli or yeast) or cell culture (mammalian or insect cells) for protein expression. Hands‑on experience with biochemical analytical techniques such as spectrophotometry, SDS‑PAGE, Western blot, SEC‑MALS, SPR, and basic mass spectrometry. Familiarity with chromatography systems (e.g., Cytiva AKTA, Agilent HPLC) and related software. Expertise in protein purification methods, including affinity, ion exchange, hydrophobic interaction (HIC), and size‑exclusion chromatography (SEC). Experience with biophysical characterization of protein complexes, including techniques like dynamic light scattering (DLS) and analytical ultracentrifugation (AUC). Experience with expression and purification of membrane proteins. Strong knowledge of protein stability, folding, and quality control for structural studies. Excellent presentation skills (written and oral) to effectively communicate research results to both scientific and cross‑functional teams. Ability to work independently, manage multiple projects, and meet deadlines in a collaborative environment. Demonstrated ability to prioritize tasks and manage multiple projects efficiently. Benefits The expected annual salary range for this role in the U.S. (excluding Puerto Rico) is posted. Actual salary will vary based on several factors including but not limited to, relevant skills, experience, and qualifications. A comprehensive employee benefits package, including a Retirement and Savings Plan with generous company contributions, group medical, dental and vision coverage, life and disability insurance, and flexible spending accounts. A discretionary annual bonus program, or for field sales representatives, a sales‑based incentive plan. Stock‑based long‑term incentives. Award‑winning time‑off plans. Flexible work models where possible. Refer to the Work Location Type in the job posting to see if this applies. Equal Opportunity Employer Amgen is an Equal Opportunity employer and will consider all qualified applicants for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, disability status, or any other basis protected by applicable law. We will ensure that individuals with disabilities are provided reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. Please contact us to request accommodation. Amgen is an Equal Opportunity employer and will consider you without regard to your race, color, religion, sex, sexual orientation, gender identity, national origin, protected veteran status, or disability status. #J-18808-Ljbffr Amgen