Research Scientist - Microbiology and Immunology

Advertising Summary: Virginia Commonwealth University (VCU) School of Medicine is a premier academic medical center located in the heart of Richmond. Accounting for almost half of VCU’s sponsored research, the School of Medicine is internationally recognized for patient care and education. Virginia Commonwealth University is an equal opportunity employer. Unit: School Of Medicine MBU Department: Microbiology and Immunology Department Summary: Located on the MCV Campus in downtown Richmond, the Department of Microbiology and Immunology is home to a vibrant community of researchers, educators and learners. With a strong history of research funding, our department strives to advance scientific knowledge in the biomedical sciences and inform medical advancements that improve patient outcomes. Our innovative studies span a broad spectrum of areas within the fields of microbiology and immunology: Allergy and autoimmunity Host-pathogen interactions and molecular pathogenesis Human microbiome science and microbial genomics Reproductive immunology T-cell therapy and vaccine development Tumor biology, cell signaling and epigenetics As educators, we are proud to provide a collaborative, collegial learning environment for our students. Faculty and postdoctoral fellows serve as dedicated mentors, providing an individualized educational experience and shaping the next generation of successful scientists. Duties & Responsibilities: A research scientist position (contingent on extramural funding) is available in the laboratory of Dr. Jason A. Carlyon in the Department of Microbiology and Immunology at Virginia Commonwealth University. The successful candidate will participate in NIH-funded projects studying the obligate intracellular bacterial pathogens, Orientia tsutsugamushi and Anaplasma phagocytophilum. Projects include developing genetic tools for O. tsutsugamushi; examining how O. tsutsugamushi modulates multiple host cell processes including immunity, membrane trafficking, cell cycle, apopotosis, lipid signaling, epigenetic gene regulation, and the ubiquitin-proteasome system; examining how A. phagocytophilum-host cell interactions drive bacterial binding, invasion, fitness, intracellular survival, immunity evasion, parasitism, and egress. Highly motivated, hard-working, and technically skilled applicants with relevant experience are welcomed to apply. Core Responsibilities: Maintenance of Orientia tsutsugamushi and Anaplasma phagocytophilum infected cultures Maintain uninfected, O. tsutsugamushi infected, and A. phagocytophilum infected cell lines under sterile conditions on a bi-weekly basis Assessment of O. tsutsugamushi molecular pathogenesis Involves purifying O. tsutsugamushi bacteria from infected cells, contributing to the development of genetic tools for O. tsutsugamushi, performing infection assays under various conditions, assessing infectivity in naïve cells. Diligently keep laboratory notebook and electronic record on server of all experiments Assessment of A. phagocytophilum interactions with eukaryotic host cells and mammalian hosts Will include all or some of the following: assess A. phagocytophilum adhesin-receptor interactions and how this promote host cell signaling, effector delivery, cytoskeletal rearrangement, and bacterial entry; analyze exosome protein, nucleotide, and lipid profiles; assess A. phagocytophilum modulation of exosome content and effector function; assess A. phagocytophilum pathogenesis using the mouse model. Diligently keep laboratory notebook and electronic record on server of all experiments Lab management Mentor and train junior scientists. Contribute to daily lab management and maintenance Writing manuscripts, grants, abstracts Write and edit drafts of manuscripts for publication. Write/contribute data for extramural grant proposals. Writing for both grants and abstracts is expected to be at or near principal investigator quality and maturity. Write abstracts of research and present at scientific conferences Demonstrated ability to work in and foster an environment of respect, professionalism and civility with a population of faculty, staff, and students from all backgrounds and experiences, or a commitment to do so as a staff member at VCU Qualifications: Minimum Qualifications (1) PhD or MD/PhD, (2) At least four years of postdoctoral experience and a proven publication record, (3) Experience in working with obligate intracellular bacterial pathogens, (3) Strong background in cell biology, microscopy, biochemistry, molecular biology, and bacterial genetics. (4) Candidates are expected to be very motivated for career development, committed to driving federally funded high impact and high-quality research, and must aggressively pursue extramural funding in the form of NIH R-awards or comparable. (5) Candidates must be able to work independently, be willing to mentor and train junior scientists, and share duties with other staff members in running lab logistics on a daily basis. (6) Candidates are expected to have excellent manuscript and grant writing skills and be extremely committed to becoming independent in these skills. (7) Candidates are expected to demonstrate excellent oral communication skills. Preferred Qualifications

• 1) Experience in working with/cultivating Orientia tsutsugamushi and Anaplasma phagocytophilum in eukaryotic tissue culture.
• 2) Experience in developing genetic tools for O. tsutsugamushi and/or A. phagocytophilum.
• 3) Experience in using confocal microscopy, electron microscopy, structured illumination microscopy, immunofluorescence microscopy, and live cell imaging to study obligate intracellular bacteria-host cell interactions.
• 4) Experience in using Fiji, other imaging software, and writing custom scripts to achieve cutting-edge image analysis.
• 5) Experience in studying obligate intracellular bacterial development, preferably Anaplasma, Orientia, and related pathogens such as Chlamydia.
• 6) Experience performing infection assays using intracellular bacterial pathogens.
• 8) Transfection, siRNA, CRISPR.

Salary Range: $75,000-$78,000 Benefits : All full-time university staff are eligible for VCU’s robust benefits package that includes comprehensive health benefits, paid annual and holiday leave, generous tuition benefits, retirement planning and savings options, tax-deferred annuity and cash match programs, employee discounts, well-being resources, abundant opportunities for career development and advancement, and more. FLSA Exemption Status: Exempt Hours per Week: 40 Restricted Position: This is a restricted position with no set end date; continued employment is dependent upon project need, availability of funding, and performance. ORP Eligible: Yes Flexible Work Arrangement: Fully Onsite University Job Title: Research Scientist 1-3 (33241-33243) Contact Information: Contact Name: Jason Carlyon, Ph.D. Contact Email: View email address on click.appcast.io #J-18808-Ljbffr Virginia Commonwealth University