UNIV- Research Specialist II- Department of Regenerative Medicine

Job Description Summary The individual will be under supervision of the PI, will participate in routine experiments and the maintenance of a basic biomedical science laboratory, with emphasis on maintenance and differentiation of human pluripotent stem cells, histology and immunohistochemistry, light microscope imaging and biochemical assays. These duties will be utilized to assist in the generation and characterization of novel models of human colonic diseases. The individual will also assist the PI to select and order reagents as well as help in setting up the lab. Entity Medical University of South Carolina (MUSC - Univ) Worker Type Employee Worker Sub-Type Research Grant Cost Center CC001060 COM REG MED Administration CC Pay Rate Type Salary Pay Grade University-GEN08 Pay Range 46,206.00 - 62,322.00 - 78,540.000 Scheduled Weekly Hours 40 Work Shift Job Description Job Description: The individual will be under supervision of the PI, will participate in routine experiments and the maintenance of a basic biomedical science laboratory, with emphasis on maintenance and differentiation of human pluripotent stem cells, histology and immunohistochemistry, light microscope imaging and biochemical assays. These duties will be utilized to assist in the generation and characterization of novel models of human colonic diseases. The individual will also assist the PI to select and order reagents as well as help in setting up the lab. Proposed Job Duties Designing and planning routine experiments, including optimization of daily experimental protocols, selection and ordering reagents and lab supplies. Maintaining an accurate and timely experimental records. Percent of Total Time: 30 Share responsibility for maintenance of human pluripotent stem cells and human intestinal and colonic organoids including, passaging, differentiation and maintenance of 3d cultures. Percent of Total Time: 30 Histology and immunohistochemistry, including tissue collection, processing and embedding, sectioning, staining, and imaging. Percent of Total Time: 20 Basic molecular biology and biochemistry experiments: DNA isolation, genotyping, real-time PCR, western blotting. Percent of Total Time: 15 Participate in animal protocol drafting, and scientific manuscript preparation. Percent of Total Time: 5 Bachelor's degree in biology, chemistry, biochemistry, molecular biology or other life science is preferred. Strong organizational, record keeping, and interpersonal communication skills required. Preference will be given to candidates with experience of various laboratory techniques including PCR and recombinant DNA work, western blotting, cell culture, histology and immunostaining, and microscopic imaging. Prior experience in lab management roles is preferred. Must be able to perform minimal mouse work. Experience with small animal surgery is not required but would be a plus. Employee is supervised by the PI but expected to conduct experiments, collect and record data with decent level of independence and discretion. Work is conducted in a basic biomedical laboratory that focuses on disease modeling using human pluripotent stem cell derived 3d colonic organoids. Additional Job Description Minimum Requirements: A bachelor's degree in chemistry, biology, or other natural, life or health care science and one year work experience directly related to the assigned research area. Physical Requirements: (Note: The following descriptions are applicable to this section: Continuous - 6-8 hours per shift; Frequent - 2-6 hours per shift; Infrequent - 0-2 hours per shift) Ability to perform job functions in an upright position. (Frequent) Ability to perform job functions in a seated position. (Continuous) Ability to perform job functions while walking/mobile. (Frequent) Ability to climb, using step stool. (Infrequent) Ability to work in temperature extremes (32° F to 100° F) when required due to assignments in cold rooms and/or foreign countries. (Infrequent) Ability to work in confined/cramped spaces. (Continuous) Ability to bend at the waist. (Frequent) Ability to twist at the waist. (Frequent) Ability to fully use both hands/arms. (Continuous) Ability to perform repetitive motions with hands/wrists/elbows and shoulders. (Frequent) Ability to fully use both legs. (Frequent) Ability to reach in all directions, stretch, bend, while moving items/materials from both sitting and standing positions. (Frequent) Ability to maintain good olfactory sensory function/distinction. (Continuous) Ability to lift materials up to 20 lbs., unassisted. (Infrequent) Ability to lift and carry 4 liter waste material bottles a distance of 30 feet, unassisted. (Frequent) Ability to lift and carry 25-30 lbs. jugs of liquid a distance of 100 feet, unassisted. (Infrequent) Ability to lift file boxes, from floor level to counter tops, unassisted. (Frequent) Ability to push/ Ability to lift 40 lbs. centrifuge rotors from floor level to height of 40 inches, then do #J-18808-Ljbffr Medical University Hospital Authority (MUHA)