Research Technician II

Overview Fred Hutchinson Cancer Center is an independent, nonprofit organization providing adult cancer treatment and groundbreaking research focused on cancer and infectious diseases. Based in Seattle, Fred Hutch is the only National Cancer Institute-designated cancer center in Washington. With a track record of global leadership in bone marrow transplantation, HIV/AIDS prevention, immunotherapy and COVID-19 vaccines, Fred Hutch has earned a reputation as one of the world’s leading cancer, infectious disease and biomedical research centers. Fred Hutch operates eight clinical care sites that provide medical oncology, infusion, radiation, proton therapy and related services, and network affiliations with hospitals in five states. Together, our fully integrated research and clinical care teams seek to discover new cures to the world’s deadliest diseases and make life beyond cancer a reality. At Fred Hutch we value collaboration, compassion, determination, excellence, innovation, integrity and respect. Our mission is directly tied to the humanity, dignity and inherent value of each employee, patient, community member and supporter. Our commitment to learning across our differences and similarities make us stronger. We seek employees who bring different and innovative ways of seeing the world and solving problems. A Research Technician II position is available to work in the Collisson Lab in the Human Biology Division to focus on lung or pancreatic cancer using a combination of genetically engineered mouse models, cell lines, and clinically derived samples. The ideal candidate will be able to independently develop, guide, and complete research projects. Responsibilities The Research Technician II will be responsible for performing standard and investigative laboratory work in support of scientific research by performing bench-level experiments, operating analytical instruments, recovering, compiling and verifying the accuracy of research data, performing calculations, and preparing charts, graphs and summaries. The lab uses standard molecular biology techniques and tissue culture as well as mouse models. Job Duties Perform experiments at the bench level to support investigative work Prepare and maintain laboratory reagents, stocks, cultures or cell lines Perform and modify documented protocols and assays Maintain laboratory records and compile data on experiments and assays Interpret and report on experimental results Perform statistical analysis of test results and prepare written reports on work performed Operate and carry out basic maintenance of laboratory equipment Order and maintain appropriate levels of supplies May supervise work-study students Participate in lab meetings and journal clubs Perform other duties as required Qualifications MINIMUM QUALIFICATIONS: Bachelor's degree in a related field 2 years of experience working in a lab outside of school coursework

PREFERRED QUALIFICATIONS:

Demonstrated ability to work as part of a team and independently. Strong ability to think rationally, problem solve and troubleshoot experiments. Excellent attention to details and the ability to work independently are essential to success in this position, as are good communication and organizational skills. The candidate must be self-motivated, able to multitask and to work within a very collaborative environment. Prepare sequencing libraries from DNA (and, where applicable, RNA-derived cDNA) samples using established kits and protocols. Execute fragmentation, end-repair, adapter ligation, size selection, and PCR amplification steps; perform bead-based cleanup and normalization. Highly proficient in a variety of molecular and cellular biology techniques, and have strong fundamental knowledge of cancer genetics and epigenetics Molecular biology techniques, including molecular cloning, nucleic acid isolation, PCR, and protein expression analysis. Experience perform nucleic acid quality and quantity assessment. Experience performing gene transfer studies using lentiviral infection of human cell cultures Knowledge in computational processing of RNA and DNA sequencing files Experience primary and established immortalized cell cultures Experience establishing and maintaining mouse colonies The hourly pay range for this position is from $27.01 to $38.45 and pay offered will be based on experience and qualifications.Although Fred Hutch is not sponsoring most H-1B visas at this time, candidates who already hold an H-1B sponsored by another organization and are currently in the U.S. may be eligible for this position. Benefits medical/vision dental flexible spending accounts life disability retirement family life support employee assistance program onsite health clinic tuition reimbursement paid vacation (12-22 days per year) paid sick leave (12-25 days per year) paid holidays (13 days per year) paid parental leave (up to 4 weeks) Additional Information We are proud to be an Equal Employment Opportunity (EEO) and Vietnam Era Veterans Readjustment Assistance Act (VEVRAA) Employer. We do not discriminate on the basis of race, color, religion, creed, ancestry, national origin, sex, age, disability (physical or mental), marital or veteran status, genetic information, sexual orientation, gender identity, political ideology, or membership in any other legally protected class. We desire priority referrals of protected veterans. If due to a disability you need assistance/and or a reasonable accommodation during the application or recruiting process, please send a request to Human Resources at View email address on click.appcast.io or by calling View phone number on click.appcast.io. #J-18808-Ljbffr Fred Hutchinson Cancer Research Center