NGS Scientist

Job Description

Job Description

Job Title: NGS Scientist
Job Location: Boston, MA
Job Duration: 6 Months
Work shift : 1st Shift
Pay Rate: $48 - $57/Hour

• Execute NGS library preparation (e.g., bulk RNA, scRNA, snRNA sequencing), quantification, and sequencing workflows to support the CMR team.
• Internalize and develop new NGS workflows to support evolving needs.
• Develop and adhere to standard operating procedures for workflows to ensure consistent production of high-quality data.
• Work in the sequencing space cross-functionally, with the ability to manage multiple requests and queues.
• Deliver NGS data with clear expectations of timelines to multiple teams and promptly update stakeholders of run completion.
• Effectively manage inventory of DNA, RNA, libraries, and sequencing reagents.
• Perform additional related tasks (i.e., RNA extraction, qPCR/ddPCR) as needed to help the team meet goals and deliverables.
• Maintain detailed documentation of NGS run performance and scientific work in an electronic notebook.
• Participate in assessment of sample prep and sequencing workflows at other sites (Indianapolis and/or contract research organizations) to enable flex capacity when demand is high ( no travel required ).

• B.S. and/or M.S. in biological or biochemical sciences (or a related field) with 3-5 years of laboratory research experience [YK1] .
• Molecular biology mastery of nucleic acid extraction and purification, quantification, NGS library preparation/QC, and qPCR.
• Strong background in NGS technologies and their applications, including quantification, sequencing, and data QC (preferably Illumina). Direct experience with RNA-seq (bulk) and amplicon-seq.
• Direct experience with single-cell and single-nucleus RNA-seq library preparation is required. Candidates must be familiar with multiple scRNA-seq/snRNA-seq platforms (e.g., 10X Genomics Chromium, Illumina PIP-seq, ScaleBio, or Parse Biosciences).
• Additional Skills/Preferences:
• Familiarity with CRISPR-based gene editing and high-throughput screening is a plus.
• Experience with automation is a plus (Mantis, Integra systems, Liquid Handlers, SPTLabtech Firefly, Tecan, etc).
• Strong organizational, time management, and communication skills.
• Experience with documentation in electronic notebook software.