Biologist

Technical/Scientific Skills
Expertise in the following is required:

• Aseptic cell culture techniques with mammalian cells
• Transient transfections and generation of stable cell lines
• Development of cell-based functional assays, biochemical and enzymatic assays, and screening of novel therapeutics in 96-/384-well plate formats at single concentrations or in concentration/response formats
• Knowledge of basic pharmacology, including concentration/response curves, determination of binding affinity, potency, Hill slope, and in vitro efficacy; mechanism-of-action studies of drugs
• Experience with colorimetric, luminescence, and fluorescence-based readouts, including HTRF
• Proficiency operating plate readers, liquid handling robots, and assay automation systems
• Design, development, and optimization of in vitro enzymatic assays such as ADP-Glo and Kinase-Glo assays
• Gene expression studies using qPCR, western blot, and FACS techniques

Soft Skills

• Strong oral and written communication skills with the ability to work independently and collaboratively
• Accurate record keeping and independent data analysis skills
• Ability to manage multiple priorities, timelines, and project goals with strong attention to detail
• Quick learner with the ability to adapt to new technologies and challenges

Minimum Qualifications

• B.S. and/or M.S. in Biology, Pharmacology, Biochemistry, or related field
• 8-10 years of laboratory experience

Additional Skills/Preferences
Experience in the following areas is preferred:

• Native cell lines, primary cell cultures, iPSCs, co-cultures, and 3D cultures
• Industry experience
• Exposure to multiple therapeutic target classes, including membrane receptors (such as GPCRs), enzymes, and transporters, as well as multiple therapeutic modalities including small molecules, peptides, antibodies, and siRNAs
• Experience with ELISA assays
• Working knowledge or background in cardiometabolic diseases

Equal Opportunity Employer / Disabled / Protected Veterans

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For temporary assignments lasting 13 weeks or longer, AllSTEM Connections is pleased to offer major medical, dental, vision, 401k and any statutory sick pay where required.

We are committed to working with and providing reasonable accommodations to individuals with disabilities. If you need a reasonable accommodation for any part of the employment process, please contact your staffing representative who will reach out to our HR team.

AllSTEM Connections participates in the E-Verify program in certain locations as required by law. Learn more about the E-Verify program.

We also consider for employment qualified applicants regardless of criminal histories, consistent with legal requirements, including, if applicable, the City of Los Angeles' Fair Chance Initiative for Hiring Ordinance. Pursuant to applicable state and municipal Fair Chance Laws and Ordinances, we will consider for employment-qualified applicants with arrest and conviction records, including, if applicable, the San Francisco Fair Chance Ordinance. For Los Angeles, CA applicants: Qualified applications with arrest or conviction records will be considered for employment in accordance with the Los Angeles County Fair Chance Ordinance for Employers and the California Fair Chance Act.

Additional Skills

(none specified)

AllSTEM Representative Contact Info

Account Executive:

Broughton

Branch Phone:

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Location:

Ontario, CA