Research Associate I - Genome Engineering

Research Associate I - Molecular Anatomy - Genome Engineering The mission of the Allen Institute is to understand the principles that govern life and to advance health. Our creative and multi-dimensional teams focus on answering some of the biggest questions in bioscience. We accelerate foundational research, catalyze bold ideas, develop tools and models, and openly share our science to make a broad, transformational impact on the world. The mission of the Allen Institute for Neural Dynamics is focused on fundamental discoveries in systems neuroscience. We are interested in how the brain builds our understanding of the complex world to guide the flexible behaviors that address our biological needs. The answers will be in terms of defined neuron types and circuits interacting across the whole brain and body. We will develop next-generation methods and theories and employ a team-based approach to discovery neuroscience. Knowledge, data, models, and tools will be widely shared to support the development of therapies for brain disorders. We are seeking a Research Associate to be part of the Proteomics team at the Allen Institute for Neural Dynamics. As a member of this team, the Research Associate will be contributing to efforts to enable precise and efficient genome engineering in the mouse brain. In recent years, there have been rapid advances in spatial transcriptomics (i.e. imaging of mRNA) but efforts to localize proteins have lagged far behind. By adopting and optimizing state-of-the-art genome engineering techniques, our team will systematically catalog the subcellular distribution of all proteins in the brain – including uncharacterized and disease-related gene products. Experimental approaches and responsibilities will involve mammalian cell culture, viral production, advanced molecular cloning including large library generation, CRISPR/Cas9 engineering, nucleic acid extraction from cells and brain tissue, PCR and next-generation sequencing. Ideal candidates will have a background in biological sciences with the temperament to work collaboratively in a fast-paced team environment and will receive training in all relevant techniques. At the Allen Institute, we believe that science is for everyone – and should be open to everyone. We are dedicated to combating biases and reducing barriers to STEM careers more broadly. We also believe that science is better when it includes different perspectives and voices. We strive to make the Allen Institute a place where everyone feels like they belong and are empowered to do their best work in a supportive environment. We are an equal-opportunity employer and strongly encourage people from all backgrounds to apply for our open positions. Essential Functions Mammalian cell culture including viral packaging Nucleic acid extraction from cultured cells and brain tissue Next-generation sequencing library preparation Advanced molecular cloning techniques Follow standardized operating procedures, good laboratory practices, and regulatory requirements Maintain clear and accurate communication within and between teams Adhere to a team schedule and manage time effectively Note: Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions. This description reflects management’s assignment of essential functions; it does not proscribe or restrict the tasks that may be assigned. Required Education and Experience Bachelor’s degree in Biology, Chemistry, Neuroscience, or related discipline Preferred Education and Experience Experience with the preparation of laboratory reagents, buffers, and aliquots Experience with CRISPR/Cas9 genome engineering Work Environment Laboratory environment - possible exposure to chemical, biological, or other hazardous substances Exposure to and handling of laboratory animal tissue This may include wearing personnel protective equipment (PPE) - including wearing respiratory protection for extended periods of time Exposure to Biosafety Level 2 organisms, such as recombinant viruses Physical Demands Fine motor movements in fingers/hands to operate computers and other office equipment; repetitive motion with lab equipment Stooping, bending, crouching Reaching, or climbing ladders Position Type/Expected Hours of Work This role is currently working onsite and is expected to work onsite for the majority of working hours. The primary work location for this role is 615 Westlake Ave N., with the flexibility to work remotely on a limited basis. Additional Comments **Please note, this opportunity does not offer work visa sponsorship** Annualized Salary Range $54,250 - $66,450* * Final salary depends on required education for the role, experience, and level of skills relevant to the role, along with work location, where applicable. Benefits Employees (and their families) are eligible to enroll in benefits per eligibility rules outlined in the Allen Institute’s Benefits Guide. These benefits include medical, dental, vision, and basic life insurance. Employees are also eligible to enroll in the Allen Institute’s 401k plan. Paid time off is also available as outlined in the Allen Institutes Benefits Guide. Details on the Allen Institute’s benefits offering are located at the following link to the Benefits Guide: It is the policy of the Allen Institute to provide equal employment opportunity (EEO) to all persons regardless of age, color, national origin, citizenship status, physical or mental disability, race, religion, creed, gender, sex, sexual orientation, gender identity and/or expression, genetic information, marital status, status with regard to public assistance, veteran status, or any other characteristic protected by federal, state or local law. In addition, the Allen Institute will provide reasonable accommodations for qualified individuals with disabilities. #J-18808-Ljbffr