Sr. Research Associate, Protein Engineering

Sarmal, Inc. is a San Diego–based biotechnology company developing next-generation genomics and molecular detection platforms. Our work integrates molecular biology, chemistry, and advanced systems engineering to enable faster, more precise, and more accessible genomic insights. We are a highly collaborative, R&D-driven team operating in a fast-paced, early-stage environment. Our scientists and engineers work across disciplines to solve complex technical challenges and translate innovation into scalable, real-world applications. At Sarmal, we value scientific rigor, curiosity, and a builder mindset. We are looking for individuals who are energized by ambiguity, motivated by impact, and excited to help bring breakthrough technologies from concept to reality. About the Job The Senior Research Associate will execute protein engineering experiments including library generation, molecular cloning, protein expression, purification, and high-throughput screening of enzyme variants. Working closely with the Protein Engineering team members, this individual will help establish scalable screening workflows and generate high-quality experimental data to support enzyme optimization for next‑generation sequencing applications. Key Role and Responsibilities Construct mutagenesis libraries using site‑directed, saturation, and combinatorial mutagenesis techniques. Perform molecular cloning, plasmid construction, and library quality assessment. Execute high‑throughput protein engineering workflows for enzyme variant generation and screening. Express and purify recombinant proteins using bacterial expression systems. Perform automated and semi‑automated screening assays in 96‑ and 384‑well plate formats. Execute biochemical and functional assays to identify improved enzyme variants. Support development and optimization of high‑throughput screening (HTS) assays. Characterize enzyme variants using activity assays and preliminary kinetic analyses. Maintain library inventories and experimental databases. Analyze screening results and summarize findings for project teams. Troubleshoot cloning, expression, purification, and screening workflows. Collaborate with Protein Engineering, Biochemistry, Computational Biology, and Automation teams. Maintain detailed laboratory notebooks and contribute to SOP development. Qualifications Industry experience in protein engineering, molecular biology, or enzyme engineering. Hands‑on experience generating mutagenesis libraries and recombinant DNA constructs. Familiarity with protein engineering workflows, including directed evolution and library generation. Experience with high‑throughput screening (HTS) platforms and automated liquid handling is highly desirable. Experience with molecular cloning techniques, including Gibson Assembly, Golden Gate Assembly, PCR, and site‑directed mutagenesis. Experience expressing recombinant proteins in bacterial systems (e.g., E. coli). Familiarity with protein purification techniques including affinity chromatography and FPLC. Experience performing enzyme activity assays and screening large numbers of variants. Strong molecular biology skills including DNA preparation, sequencing verification, and plasmid construction. Excellent organizational, troubleshooting, and experimental documentation skills. Ability to work effectively in a collaborative, fast‑paced startup environment. Experience B.S. or M.S. in Molecular Biology, Biochemistry, Biotechnology, Protein Engineering, Chemical Biology, or a related field. B.S. with 5–8 years or M.S. with 2–3 years of relevant industry experience. Demonstrated experience in protein engineering or enzyme engineering laboratories. Hands‑on experience with library generation, mutagenesis, and high‑throughput screening workflows. Experience with recombinant protein expression and purification. Experience supporting directed evolution or enzyme optimization projects is strongly preferred. Experience with polymerases, nucleic acid enzymes, or sequencing technologies is a strong plus. Why Join Sarmal Work on cutting‑edge genomics and molecular detection technologies Collaborate with a deeply technical, cross‑functional team Contribute to foundational platform development at an early stage Have visibility into and impact on core technical and strategic decisions Help shape the infrastructure, culture, and future direction of the company Salary Range The anticipated salary range for this position is $72K-$85K, depending on experience and qualifications. Final compensation will be determined based on a variety of factors including, but not limited to, location, relevant experience, skill set, and internal equity considerations. Sarmal offers a competitive and comprehensive benefits package, including: Medical (100% employee), dental, and vision coverage Company‑paid life insurance Generous paid time off Holiday recharge period in addition to observed holiday schedule Access to additional voluntary benefits, employee perks, and discount programs Access to onsite gym We are committed to supporting the well‑being of our employees and providing a strong foundation for both professional and personal success. Additional Information Applicants must have legal authorization to work in the United States without the need for current or future employer sponsorship. All new hires will be required to verify identity and eligibility to work in the United States, and complete Form I‑9 upon hire. We do not accept unsolicited resumes from search firms. Any resume submitted without a signed agreement will be considered unsolicited, and Sarmal will not be obligated to pay any fees. Sarmal is an equal opportunity employer committed to building a diverse and inclusive team. We do not discriminate based on any protected characteristic under applicable federal, state, or local law. If you require reasonable accommodation during the application or interview process, please contact the Human Resources team at View email address on click.appcast.io. We are committed to ensuring equal access and opportunity for all applicants. Join us in advancing the next generation of genomic technologies and shaping the future of precision health. #J-18808-Ljbffr Sarmal