Scientist II, Delivery for Large Gene Insertion

Profluent seeks a Scientist II to lead development of non-viral delivery methods for large gene insertion using recombinase-based approaches. The role focuses on solving fundamental challenges in nucleic acid donor template delivery for mammalian cells, with opportunities to work across AI, protein design, and gene editing teams.

Responsibilities

• Lead development, evaluation, and benchmarking of non-viral donor template delivery methods for recombinase-based large gene insertion
• Design and execute hypothesis-driven experiments to identify and overcome barriers to donor template stability, trafficking, and nuclear availability
• Develop quantitative assays connecting donor template delivery measurements to insertion efficiency and fidelity
• Analyze data to inform go/no-go decisions and present conclusions to cross-functional stakeholders
• Validate delivery approaches in therapeutically relevant cell-based and in vivo systems with translational teams

Requirements

• PhD in molecular biology, biochemistry, bioengineering, or related field plus 2-4 years industry or academic experience
• Expertise in gene editing, nucleic acid delivery, or protein engineering with proven track record of solving impactful problems
• Strong hands-on experience in molecular cloning, nucleic acid construct design, and mammalian cell engineering
• Deep understanding of nucleic acid delivery barriers in mammalian cells and therapeutic applications
• Extensive experience with NGS-based assays for evaluating genome editing outcomes
• Demonstrated ability to develop mechanistic cell-based assays and troubleshoot complex biological systems
• Proven success leading multifaceted research projects independently with strong communication skills