Laboratory Technician III

As a community, the University of Rochester is defined by a deep commitment to Meliora - Ever Better. Embedded in that ideal are the values we share: equity, leadership, integrity, openness, respect, and accountability. Together, we will set the highest standards for how we treat each other to ensure our community is welcoming to all and is a place where all can thrive. Job Location (Full Address): 601 Elmwood Ave, Rochester, New York, United States of America, 14642 Opening Worker Subtype: Regular Time Type: Full time Scheduled Weekly Hours: 40 Department: 400088 Pediatrics M&D Neonatology Work Shift: Range: UR URG 106 H Compensation Range: $21.36 - $29.90 The referenced pay range represents the minimum and maximum compensation for this job. Individual annual salaries/hourly rates will be set within the job’s compensation range, and will be determined by considering factors including, but not limited to, market data, education, experience, qualifications, expertise of the individual, and internal equity considerations. Responsibilities Performs complex laboratory assignments, planning and conducting experiments of limited scope or a portion of a larger and more diverse project. Leads tasks associated with research activities and lab support for lower-level technicians. Serves as unit operations liaison to ensure compliance with departmental or University policies, procedures, and defined internal controls. Ensures accountability and stewardship of University resources (operational, financial, and human) in compliance with departmental standards and procedures. General Purpose This position requires active participation in research laboratory activities. This individual will participate in basic science laboratory procedures including routine experiments using both cell culture and transgenic mouse models of erythropoiesis. Research will require experience with cell culture, mouse breeding/genotyping, FACS analyses, polymerase chain reaction, cloning techniques, tissue culture procedures, and fluorescent antibody detection. This position will involve the generation and maintenance of cell lines, handling mice, and analyzing erythropoiesis in cell culture and animal models. Cell culture Culture mouse and human erythroblasts, including monitoring of cultures using benzidine and trypan counts Generate and maintain cell lines for analyses using Crispr genome editing Genotype cell lines Analyze cultures using flow cytometric analyses Molecular Biology Generate guide RNAs for Crispr experiments Design and perform cloning of constructs as directed Screen colonies generated via genome editing experiments using qPCR and western blot Assay mRNA and protein levels using qPCR and Western blot, respectively Research Laboratory Procedures Organizing the work stations with the necessary supplies, including assembling any necessary plastic ware Cell staining and counting for flow cytometry experiments Performing routine laboratory analyses including (but not limited to) RT-PCR, western blot, immunofluorescence, cell culture, and microscopy. Routine cleaning of instrumentation and work area per protocol Restocking of work station supplies and ordering of supplies Animal Procedures Assist with genotyping and colony maintenance Lab Meetings and Journal Clubs Participate in lab meetings and journal clubs. Keeping current in the field through pertinent seminars and literature. Other duties as assigned Qualifications Associate’s degree in appropriate discipline plus 2-3 years specialized experience in related field; or equivalent combination of education and experience required. BS/BA in biological/physical science or equivalent experience is preferred. A minimum of 1-year experience in laboratory science desired. Individuals with experience in handling mice and/or cytometry and cell culture are encouraged to apply required. Knowledge of University policy and procedures is desirable. Must have reliable/efficient transportation preferred. The University of Rochester is committed to fostering, cultivating, and preserving an inclusive and welcoming culture to advance the University’s Mission to Learn, Discover, Heal, Create – and Make the World Ever Better. In support of our values and those of our society, the University is committed to not discriminating on the basis of age, color, disability, ethnicity, gender identity or expression, genetic information, marital status, military/veteran status, national origin, race, religion/creed, sex, sexual orientation, citizenship status, or any other characteristic protected by federal, state, or local law (Protected Classes). This commitment extends to non-discrimination in the administration of our policies, admissions, employment, access, and recruitment of candidates for all persons consistent with our values and based on applicable law. #J-18808-Ljbffr