Research Technician Skilled In Mouse Work

Position Requirements

Overall Position Summary and Objectives	Min Education	Resume Max Pages
The primary objective of the position is to provide research support to NHGRI scientists by per-forming a variety of assignments involving the application of a full range of cell biology related laboratory methods, procedures and techniques associated with NIH biomedical research environ-ment.	Bachelor's	3

Additional Qualifications

Certifications & Licenses - Biosafety Level 1 (BSL 1)	Field of Study - Biology
Software - Powerpoint - Excel - Word - R	Skills - Animal Handling: Mouse - Manuscript writing - Tissue Sectioning Data Presentation - Primary cell culture - Western Blotting

Work Location

IC: NHGRI		Street: 10 Center Dr		Bldg: 10		Room: 10C107
City : Bethesda			State & Zip : MD 20814
Work Hours
Weekly Hours	FT: 30-40 hours per week	# Of Hours		40

Statement of Work Details

Plans, executes, and arranges laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

- Collaboratively work with mouse models of FSASD and GNE in NHGRI animal facility, activities include mouse breeding, observation, tissue collection, testing of therapeutics and documentation of breeding/tissue collection logistics. 1

- Provide research support to NHGRI scientists with planning, developing and implementing la-boratory experiments and protocols;assist in coordinating communication with the Research Consortium on Free Sialic Acid Storage Disorder (FSASD). 2

- Perform laboratory technologies pertaining to molecular biology, biochemistry, glycobiology and cell biology. Such techniques includesterile cell culture, PCR, qRT-PCR, DNA/RNA as-says, cloning, protein assays, glycobiology tools, lectin technologies, antibody techniques. 3

- Operate a variety of laboratory instruments, including but not limited to (confocal)-microscopes, spectrophotometer/plate reader, otherimaging equipment. 4

Operates laboratory machines and instruments; and prepares specimens and equipment for use.




- Work with staff on processing specimens for embedding according to protocols.

Performs sample management and storage.

- Maintain inventory system and sample storage according to outlined guidelines.

Analyzes, documents and reports on all procedures performed.




- Convey and discuss research results with team lab members. 5

Maintains inventory of laboratory supplies.

- Ensure that supplies and consumables are ready and on hand as necessary for maximum efficiency in the laboratory.

Cleans and sterilizes instruments, glassware and equipment.

- Work with staff to cover glassware washing, media preparation, and autoclaving.

Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

- Plan and execute experiments for in vitro and in vivo studies, including cell culture, Western blotting and immunofluorescence/immunohistochemistry.

Maintains inventory of laboratory supplies and initiates purchase orders.

- Establish and maintain a record of laboratory supply usage.

Performs routine equipment maintenance.

- Perform laboratory maintenance tasks, including scheduling liquid waste container pickup.

Plan execute and arrange laboratory experiments, new techniques and procedures; and performs laboratory techniques relating to scientific research.

- Perform fundamental procedures required for research projects.

- Perform routine experiments and tests, including tissue culture, cell separation, protein, and quantitative and qualitative analysis.

- Perform primary cell culture techniques and immunohistochemistry.

- Perform a variety of laboratory techniques and procedures.

Analyzes, documents and reports on all procedures performed and produces detailed reports for use by senior staff.




- Provide experimental progress updates on procedures performed in the laboratory.

- Prepare reports and summaries based on results and findings.

- Present results and conclusions in written, tabular and graphic form.

- Prepare accumulated data and conclusions for publication in professional journals.

- Write reports detailing experiments and data analysis; produce publication quality graphs.

- Collect, analyze and interpret data; prepare summaries and reports based on results and findings.

Performs advanced equipment maintenance.

- Perform laboratory maintenance tasks, including scheduling liquid waste container pickup.

Trains staff in performing routine laboratory tests and procedures.

- Provide technical help and expertise to lab members.

Provides insights and expertise and recommends new strategies and approaches.

- Develop and assist in new assays for application based on requests from investigators