Postdoctoral Scientist - Gene Therapy Biodistribution & Molecular Biology (CNS)

Position Overview We are looking for a highly motivated Postdoctoral Scientist to join the Drug Delivery and Connected Systems (DDCS) organization, focusing on CNS gene therapy biodistribution and molecular bioanalysis. The role supports the optimization and translational advancement of gene therapy platforms for neurological and neurodegenerative diseases. The successful candidate brings deep expertise in biodistribution assay development, molecular biology, quantitative bioanalysis, and works at the interface of in vivo CNS pharmacology, vector biology, and translational science. Biodistribution & Molecular Bioanalysis Lead the design, development, optimization, and execution of biodistribution assays to assess AAVs, LNPs, siRNAs, transgene expression, and persistence across CNS and peripheral tissues. Apply a broad toolkit of molecular and bioanalytical techniques, including PCR-based methods (PCR, qPCR, ddPCR, stem-loop PCR, vg/dg dose quantification), ELISA and immunoassays for protein expression, LC–MS–based quantitative bioanalysis, Immunohistochemistry (IHC), tissue-based spatial analyses, and next-generation sequencing (NGS). Ensure assays are fit-for-purpose, sensitive, reproducible, and aligned with translational gene therapy objectives. Develop off‑target analysis and DRG toxicology assays. Experimental Design, Troubleshooting, and Scientific Rigor Independently design and execute CNS gene therapy–focused biodistribution studies, applying sound experimental design and Design of Experiments (DoE/DOR) principles. Diagnose and troubleshoot complex technical challenges related to vector detection, tissue processing, assay performance, or data variability. Maintain meticulous experimental documentation, ensuring traceability, data integrity, and compliance with DDCS best practices. CNS Gene Therapy & Translational Insight Apply foundational knowledge of CNS biology, molecular biology, and biochemistry to interpret biodistribution and expression data in a therapeutic context. Integrate biodistribution results with pharmacokinetics and pharmacodynamics (PK/PD) to inform dose selection, delivery route optimization, and therapeutic index assessment. Contribute to translational strategy discussions by linking molecular readouts to functional, anatomical, and disease-relevant endpoints. In‑Vivo Study Oversight & External Collaboration Interface with external CROs to design, execute, and oversee in‑vivo gene therapy biodistribution studies, including work in higher vertebrate CNS models. Provide scientific oversight for tissue collection, sample processing, and downstream molecular analyses conducted internally or externally. Ensure CRO outputs meet DDCS scientific standards, timelines, and data-quality expectations. Data Analysis, Visualization, and Team Communication Apply best practices for managing and analyzing large, multi-dimensional biodistribution datasets. Perform statistical analysis and generate clear, insightful data visualizations to support project teams and decision-making forums. Communicate findings effectively through presentations, written summaries, and collaborative scientific discussions. Basic Requirements PhD in Molecular Biology, Biochemistry, Pharmacology, Neuroscience, Bioengineering, or a closely related life sciences discipline. Demonstrated in‑depth experience in AAV biodistribution analysis and/or molecular bioanalytical assay development. Strong hands‑on expertise in PCR/qPCR/ddPCR, ELISA, IHC, NGS, and/or related molecular biology techniques. Solid understanding of CNS gene therapy delivery, molecular mechanisms of transgene expression, and/or tissue-specific biology. Proven ability to independently design experiments, troubleshoot technically challenging assays, and maintain rigorous scientific documentation. Highly motivated, proactive, and able to thrive in a collaborative, team‑based research environment. Authorized to work in the United States on a full‑time basis; no work‑authorization sponsorship provided. Additional Preferences Knowledge of pharmacokinetics and pharmacodynamics as applied to gene therapy biodistribution and expression. Spatial transcriptomics / RNAscope experience for CNS transgene expression mapping. Direct experience working with CNS tissues and CNS‑relevant animal models for gene therapy research. Experience integrating biodistribution data with imaging, functional, or efficacy readouts. Experience collaborating with CROs and supporting in‑vivo gene therapy studies. Familiarity with FDA (2020) long‑term follow‑up guidance and EMA biodistribution reflection papers. Knowledge of current FDA guidance on intrathecal/systemic AAV safety signals. Other Information This is a fixed‑term role for one year, extendable up to four years, with eligibility to apply for full‑time positions upon completion. The position is based out of the Boston, MA Lilly location and requires daily, on‑site presence and lab work. Compensation and Benefits Actual compensation will depend on a candidate’s education, experience, skills, and geographic location. The anticipated wage range for this position is $58,000 – $123,200. Full‑time employees also are eligible for a company bonus (depending, in part, on company and individual performance). Lilly offers a comprehensive benefit program to eligible employees, including eligibility to participate in a company‑sponsored 401(k); pension; vacation benefits; eligibility for medical, dental, vision and prescription drug benefits; flexible benefits (e.g., healthcare and/or dependent day‑care flexible spending accounts); life insurance and death benefits; certain time‑off and leave of absence benefits; and well‑being benefits (e.g., employee assistance program, fitness benefits, and employee clubs and activities). Accommodation and EEO Lilly is proud to be an EEO Employer and does not discriminate on the basis of age, race, color, religion, gender identity, sex, gender expression, sexual orientation, genetic information, ancestry, national origin, protected veteran status, disability, or any other legally protected status. Lilly is dedicated to helping individuals with disabilities actively engage in the workforce. 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