Associate Scientist

Job Description

Job Description

Payrate: $25.00 - $28.00/hr.

Summary:
We are seeking a Research Associate to work on RNA post-transcriptional processing and regulation in human cells. The Research Associate will perform experiments, maintainaccurate records, and work closely with the project scientist to interpret results and determinethe next steps. The ideal candidate is curious, resourceful, and willing to ask questions, investigate unexpected results, and seeexperiments through to completion.

Responsibilities:

• Maintain, passage, treat, transfect, and preserve mammalian cell lines. Monitor cell health and recognize potential issues with growth, media, or contamination.
• Perform molecular biology experiments, including DNA and RNA purification, plasmid preparation, PCR, electrophoresis, and preparation of solutions and dilutions.
• Conduct RNA experiments and assays, including reporter and minigene assays, RNA isolation, RT-qPCR, and preparation of next generation sequencing libraries. Perform additional RNA or protein assays as project evolves.
• Prepare reagents, maintain supplies and equipment, and keep the laboratory organized and ready for experiments. Follow applicable site safety procedures, policies, and standard operating procedures.
• Follow experimental protocols carefully, use appropriate controls, and maintain accurate sample identities and records, including when handling large numbers of samples.
• Assess the quality of samples and experimental results. Investigate unexpected findings, troubleshoot technical problems, and discuss possible explanations and next steps with the project scientist.
• Work independently to prioritize experiments and meet timelines while collaborating with the project scientist and other members of the research group.
• Use spreadsheets, electronic laboratory notebooks, and presentation software to document experiments, track samples, organize data, and communicate results.
• Communicate clearly and respectfully with colleagues, take direction and feedback, and share methods or provide hands-on training when appropriate.

Preferred Qualifications:

• Bachelor’s degree in molecular biology, cell biology, biochemistry, genetics, or a related field, with at least 1-2 years of laboratory experience.
• Experience with mammalian cell culture.
• Experience with RNA isolation, RNA quality control, RNA sequencing library preparation, and NGS.
• Experience with one or more of the following: oligonucleotide or siRNA perturbation, reporter assays, immunoprecipitation, Western blotting, or RT-qPCR.
• Familiarity with RNA post-transcriptional processing such as splicing, translation or cleavage and polyadenylation.
• Demonstrated ability to troubleshoot experiments, interpret results in the context of a research question, communicate clearly, and work both independently and collaboratively .

Top 3 Must Have Skill Sets:

• Research/Laboratory experience in molecular biology for at least 1 year, preferably more.
• Familiarity with RNA biology, processing and regulation (splicing, translation, cleavage and polyadenylation)
• Experience with one or more of the following: oligonucleotide or siRNA perturbation, reporter assays, immunoprecipitation, Western blotting, or RT-qPCR. Preference to candidates who have experience in more or all.

Pay Transparency:  The typical base pay for this role across the U.S. is: $25.00 - $28.00 /hr . Non-exempt positions are eligible for overtime at a rate of 1.5 times the base hourly rate for all hours worked in excess of 40 in a work week, or as required by state or local law. Final offer amounts, within the base pay set forth above, are determined by factors including your relevant skills, education and experience. Full-time employees are eligible to select from different benefits packages. Packages may include medical, dental, and vision benefits, health savings accounts with qualified medical plan enrollment, 10 paid days off, 3 days paid bereavement leave, 401(k) plan participation with employer match, life and disability insurance, commuter benefits, dependent care flexible spending account, accident insurance, critical illness insurance, hospital indemnity insurance, accommodations and reimbursement for work travel, and discretionary performance or recognition bonus. Sick leave and mobile phone reimbursement provided based on state or local law. 

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