Research Scientist

Reports to: Director, Discovery Research About Our Company: We are a stealth-mode biotechnology company developing next generation complex biologics, with an initial focus on high-impact targets in immunology. Our mission is to rapidly design and globally develop highly differentiated therapies for patients with life-threatening diseases. Position Overview: We are seeking a creative, motivated and solutions-oriented cell biologist to join the discovery research team and generate the experimental biology that powers an integrated, AI-assisted discovery engine. Working at the bench and in close partnership with our computational colleagues, this individual will design and execute cell-based assays that characterize multi-specific biologics against targets in autoimmune disease and immuno‑oncology, and will produce the high-quality, structured data that closes our "lab-in-a-loop" - the iterative cycle in which computational design informs experiments and experimental results refine the next round of design. The role suits a hands-on scientist with deep experience in primary immune cell biology and functional assay development who is eager to work at the interface of wet-lab biology and computation. Key Responsibilities: Design, optimize and execute cell-based assays to characterize the activity, potency and selectivity of multi-specific biologics, including T-cell-redirected cytotoxicity and co-culture killing assays. Establish and maintain primary human immune cell systems (e.g., T cells, PBMCs) and relevant cell line models for functional and mechanistic studies. Develop quantitative readouts of immune activation and function—flow cytometry, cytokine profiling, high-content imaging—to define mechanism of action and support candidate selection. Generate structured, well-annotated datasets and maintain rigorous electronic lab notebook records so results flow cleanly back into computational models and decision‑making. Partner with computational and protein engineering colleagues to translate design hypotheses into testable experiments and to interpret results in the context of ongoing design cycles. Troubleshoot assays, implement new techniques, and contribute to a strong culture of experimental rigor and reproducibility. Present data and interpretations in team and cross‑functional research meetings. Maintain high standards of laboratory safety. Essential Qualifications: PhD in immunology, cell biology or a related discipline with 2+ years of relevant experience, or an MS/BS with 5+ years of industry experience. Hands‑on expertise culturing and manipulating primary human immune cells and cell line models. Strong experience in flow cytometry and cell‑based functional assays (e.g., cytotoxicity, proliferation, activation, cytokine release). Demonstrated ability to develop and optimize assays independently and to execute with rigor and attention to detail. Excellent record‑keeping using an ELN and a collaborative, proactive approach to teamwork. Ideal Qualifications: Experience characterizing multi‑specific biologics; familiarity with T-cell engagers (TCE) or other complex modalities is a plus. Experience with in vitro models of autoimmune disease and/or immuno‑oncology. Comfort working in a data‑rich, computationally integrated environment and enthusiasm for AI‑assisted discovery. Experience with high‑content imaging or high‑throughput assay formats. Two River is an equal opportunity employer. We are committed to building a team with a range of backgrounds, experiences and perspectives, and we evaluate all qualified applicants without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, status as a protected veteran, genetic information, or any other characteristic protected by applicable law. We provide reasonable accommodations to applicants and employees with disabilities. #J-18808-Ljbffr