Research Fellow - Lab Medicine and Pathology

The candidate will be working under the mentorship of Dr. Maia and Dr. Harrington in a joint project to define transcriptional dependencies associated with serous (p53 Aberrant) endometrial cancer. Responsibilities Cultivate mammalian cell cultures, primarily intestinal organoid/tumoroid models Derive and establish normal and cancer organoid lines from patient samples Design and conduct drug screenings and evaluate the efficacy of novel therapeutics in preclinical organoid models. Use reporter expressing organoid models to assess cell state dynamics, in response to various compounds and drug candidates, using flow cytometry analysis. Conduct a wide range of molecular biology techniques, such as DNA/RNA extraction, PCR, qPCR, cloning, western blotting and chromatin immunoprecipitation (ChiP). Interpret, present, and document experiments based on generated data to investigators Maintain and report accurate records of data Experience with genomics techniques such as ATAC-seq, RNA-seq, ChiP-seq, CUT&RUN is preferred. Experience within vivo preclinical models of oncology is also preferred. #J-18808-Ljbffr Mayo Clinic