Research Scientist/Engr 1

Field of Research Protein engineering, synthetic biology, and biochemical tool development with emphasis on optogenetically controlled RNA/DNA polymerases, programmable nucleic‑acid writing, and in vitro assay systems for polymerase activity, specificity, and error control. Purpose of the Research Project(s) To build and execute the experimental pipeline for engineering and characterizing light‑responsive RNA polymerase systems. The position will support molecular cloning, protein production, and cell‑free functional assays needed to generate sponsor‑facing milestones and publications on optogenetic polymerase control, sequence‑programmed RNA synthesis, and related protein‑sensor technologies. Job Duties and Responsibilities Molecular cloning and construct generation – 25%: Design and build plasmids for engineered T7 polymerases, optogenetic domains, protein binders, reporters, and assay controls using PCR, Gibson/Golden Gate assembly, mutagenesis, and sequence verification. Protein expression, purification, and QC – 20%: Express and purify polymerases, binders, and optogenetic protein components (primarily from bacteria) using affinity chromatography/FPLC and standard QC methods such as SDS‑PAGE, concentration assessment, and storage optimization. In vitro functional assay development and execution – 25%: Develop and run cell‑free transcription and polymerase assays, including time‑course transcription assays, one‑ or few‑base incorporation assays, and assays using modified nucleotides such as fluorescently labeled or biotinylated bases. Optogenetic characterization and readout integration – 15%: Perform light‑control experiments, fluorescence/plate‑reader assays, and other optical measurements needed to evaluate gating behavior, kinetics, and retained function of engineered constructs. Sequencing sample preparation and data/QC handoff – 10%: Prepare samples for sequencing‑based readouts, organize construct/sample metadata, and maintain clean experimental records that support downstream analysis and sponsor reporting. Project support, documentation, and collaboration – 5%: Maintain SOPs, reagent inventories, and experimental documentation; prepare figures/tables for meetings and reports; and coordinate day‑to‑day bench workflows with the PI, trainees, and collaborating groups. Visa Eligibility This position is eligible for H‑1B sponsorship. Minimum Qualifications Bachelor's Degree in Biochemistry, molecular biology, bioengineering, chem biol, or related field and one year of relevant experience laboratory research. Equivalent education and/or experience may substitute for minimum qualifications except when there are legal requirements, such as a license, certification, and/or registration. Additional Requirements Molecular cloning: PCR, Gibson/Golden Gate assembly, mutagenesis, bacterial transformation, plasmid preparation, and sequence verification. Protein production: bacterial expression, affinity purification, chromatography/FPLC workflows, and protein QC. Biochemical assays: experience with enzyme assays, transcription assays, fluorescence‑based readouts, or related in vitro functional measurements. Desire Experience with cell‑free transcription or nucleic‑acid enzyme assays. Experience using modified nucleotides (e.g., fluorescent, biotinylated, or otherwise chemically functionalized substrates). Experience with optogenetic proteins, light‑controlled assays, or photochemistry/fluorescence instrumentation. Sequencing library preparation or sample‑prep familiarity for assay readouts. Experience with protein binders, display/selections, or protein‑sensor engineering. Basic computational comfort for organizing data, plotting results, and working with standard lab analysis tools. Data quality and documentation: careful experimental record‑keeping, sample tracking, and SOP development. Communication and teamwork: ability to summarize results clearly and work closely with the PI and collaborators. Research assistantships/internships acceptable, strong hands‑on. Compensation and Position Details Pay Range Minimum: $56,448.00 annual Pay Range Maximum: $58,008.00 annual Other Compensation: - Shift: First Shift (United States of America) Type: Regular position

FTE: 100.00%

Union/Bargaining Unit: UAW Research Our Commitment The University of Washington is committed to fostering an inclusive, respectful and welcoming community for all. As an equal opportunity employer, the University considers applicants for employment without regard to race, color, creed, religion, national origin, citizenship, sex, pregnancy, age, marital status, sexual orientation, gender identity or expression, genetic information, disability, or veteran status consistent with EW Executive Order No. 81. To request disability accommodation in the application process, contact the Disability Services Office at View phone number on click.appcast.io or View email address on click.appcast.io. Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current and past employment. Disclosure is required under Washington state law. The University of Washington is an affirmative action and equal opportunity employer. All qualified applicants will receive consideration for employment without regard to any of the above categories. #J-18808-Ljbffr University of Washington