Research Associate I - ADRD

Job ID: req4603 Employee Type: nonexempt full-time Division: Applied & Development Research Program Facility: Frederick: Ft Detrick Location: PO Box B, Frederick, MD21702 USA The Frederick National Laboratory is operated by Leidos Biomedical Research, Inc. The lab addresses some of the most urgent and intractable problems in the biomedical sciences in cancer and AIDS, drug development and first-in-human clinical trials, applications of nanotechnology in medicine, and rapid response to emerging threats of infectious diseases. Accountability, Compassion, Collaboration, Dedication, Integrity and Versatility; it's the FNL way. Program Description The In Vivo Evaluation Group, Biological Testing Branch (BTB) of the Developmental Therapeutics Program (DTP), Division of Cancer Treatment and Diagnosis (DCTD) is establishing both in vitro and in vivo tumor lines from patient biopsies from DCTD sponsored clinical trials with the goal of developing models for NCI’s Patient Derived Models Repository (PDMR). The PDMR is a repository of patient-derived models that include in vivo tumor fragments, in vitro tumor lines (2D and 3D), cancer associated fibroblasts, and DNA/RNA isolates. The goal of the PDMR is to distribute well validated and highly characterized material to researchers worldwide. Key Roles/Responsibilities Aid in the daily maintenance and cryopreservation of the various cultures grown in different media Establish organoid cultures from patient samples and from tumors isolated from mice Prepare cultures for the various validation assays (flow cytometry, cell sorting, Q RT PCR, soft agar clonal assays, RNA/DNA extraction, the STR identity assay, proliferation assays, etc) Prepare all required media, components and reagents used in the culture of organoid cultures Participate in evaluating the effects of different basement membrane extracts, media additives, as well as different protocols on the establishment and growth of both 2D and 3D cell lines Aid in other routine tasks as needed such as cell photography, PowerPoint presentations, tube/plate labeling, cell deliveries/pick-ups etc Maintain precise documentation of each 2D/3D cell line in Excel spreadsheets. Basic Qualifications Possession of a Bachelor’s degree from an accredited college or university according to the Council for Higher Education Accreditation (CHEA), or four (4) years relevant experience in lieu of degree. Foreign degrees must be evaluated for U.S. equivalency In addition to the education requirement, a minimum of two (2) years of progressively responsible experience Ability to be flexible to meet changing needs Organizational skills and database management skills Ability to work in a fast-paced environment Ability to work under Biosafety Level-2 conditions Ability to work alone and as part of a team Ability to obtain and maintain a security clearance Preferred Qualifications Knowledge of sterile eukaryotic tissue culture techniques Knowledge of organoid cell culture Job Hazards This position is subject to working with or has the potential for exposure to animals(s) and/or animal material(s) This position is subject to working with or has the potential for exposure to infectious material, requiring medical clearance and immunizations Commitment to Non-Discrimination All qualified applicants will receive consideration for employment without regard to sex, race, ethnicity, color, age, national origin, citizenship, religion, physical or mental disability, medical condition, genetic information, pregnancy, family structure, marital status, ancestry, domestic partner status, sexual orientation, gender identity or expression, veteran or military status, or any other basis prohibited by law. Leidos will also consider for employment qualified applicants with criminal histories consistent with relevant laws. Pay And Benefits Pay and benefits are fundamental to any career decision. That's why we craft compensation packages that reflect the importance of the work we do for our customers. Employment benefits include competitive compensation, Health and Wellness programs, Income Protection, Paid Leave and Retirement. More details are available here 58,200.00 - 83,353.00 USD The posted pay range for this job is a general guideline and not a guarantee of compensation or salary. Additional factors considered in extending an offer include, but are not limited to, responsibilities of the job, education, experience, knowledge, skills, and abilities as well as internal equity, and alignment with market data. The salary range posted is a full-time equivalent salary and will vary depending on scheduled hours for part time positions #J-18808-Ljbffr