Scientist

Scientist – Viral Culture & Molecular Biology Work Location: Montgomery County, Maryland Summary: Seeking a hands‑on scientist with expertise in viral culture and molecular biology to support manufacturing and research operations. The role requires independent work in a laboratory setting, focusing on viral systems and molecular techniques. Responsibilities: Conduct recombinant viral culture production using advanced transfection methods such as electroporation and Lipofectamine. Manage and culture viral systems, including HIV and EBV, for manufacturing purposes while adhering to biosafety standards. Grow and maintain both adherent and suspension cell lines in various formats for experimental and production needs. Apply molecular biology techniques, including plasmid purification, gel electrophoresis, and RNA transcription, to support laboratory workflows. Perform nucleic acid quantitation and integrity analysis using Qubit and other relevant technologies. Design, execute, and interpret real-time qPCR and digital PCR assays for research and production applications. Work independently with minimal supervision, ensuring meticulous documentation and record-keeping of laboratory activities. Comply with GMP and/or GLP standards and maintain a safe laboratory environment in accordance with biosafety protocols. Qualifications: BS/MS in Biology, Biochemistry, Molecular Biology, or a related field with 3-5 years of relevant laboratory experience. Demonstrated hands‑on experience with viral culture systems, particularly HIV and EBV. Proficiency in recombinant viral production techniques, including electroporation and Lipofectamine-based methods. Strong skills in culturing both adherent and suspension cell lines. Experience with plasmid purification, gel electrophoresis, RNA transcription, and Qubit quantitation. Expertise in real-time qPCR and digital PCR platforms. Ability to work independently and manage multiple laboratory tasks efficiently. BSL2 laboratory experience and familiarity with biosafety protocols for handling infectious agents. Experience in a GMP manufacturing or diagnostics environment is a plus. Background in reference standards, controls, or reagent manufacturing is desirable. Familiarity with formalin-fixed cell preparation is preferred. #J-18808-Ljbffr ClinLab Solutions Group