Research Technician I

Site: The General Hospital Corporation Mass General Brigham relies on a wide range of professionals, including doctors, nurses, business people, tech experts, researchers, and systems analysts to advance our mission. As a not-for-profit, we support patient care, research, teaching, and community service, striving to provide exceptional care. We believe that high-performing teams drive groundbreaking medical discoveries and invite all applicants to join us and experience what it means to be part of Mass General Brigham. Job Summary The Stanton Laboratory in the Center for Genomic Medicine (CGM) at Massachusetts General Hospital (MGH) is seeking a motivated, enthusiastic candidate to join our research group. Our research group works with brain 3D organoid model to understand the biological complexity, target identification, and validation in neurodegenerative disease. We are seeking a highly motivated Research Technician to join our research team focused on human induced pluripotent stem-cell (iPSC) models, gene editing, cellular phenotyping, and 3D brain organoid systems. The successful candidate will play a central role in supporting projects involving iPSC culture, CRISPR-based genome editing, directed differentiation, molecular cloning, and downstream validation assays. This position is ideal for someone with strong hands-on experience in mammalian cell culture and molecular biology who is excited to contribute to a fast-paced, collaborative research environment. The technician will be expected to maintain rigorous experimental records, generate reproducible data, and assist in day-to-day laboratory operations. Does this position require Patient Care? No Perform laboratory experiments following established protocols and procedures. Prepare and handle samples, reagents, and solutions, ensuring accuracy and adherence to safety guidelines. Operate and maintain laboratory equipment and instruments, conducting routine calibration and troubleshooting as needed. Record experimental data accurately and maintain organized records of procedures, protocols, and results. Compile and analyze data using appropriate statistical and analytical methods. Assist in data interpretation and presentation, contributing to research findings and reports. Key responsibilities: • Maintain and expand human iPSC lines under sterile culture conditions • Conduct CRISPR-based gene editing workflows in iPSCs, including transfection/nucleofection, lentiviral transduction, clone handling, and expansion • Support generation, validation, and maintenance of edited cell lines

• Perform directed differentiation of iPSCs into relevant downstream cell types
• Generate, culture, and maintain 3D brain organoids • Assist with optimization

and routine handling of organoid-based workflows • Validate differentiated cells and organoid-derived cultures using - Flow cytometry, Immunofluorescence staining and imaging, and perform functional assay as required • Carry out molecular cloning tasks such as plasmid preparation, bacterial transformation, restriction digest analysis, Gibson assembly, PCR, and construct verification • Assist with genotyping, sequencing library preparation, and clone validation • Maintain accurate, organized, and detailed lab notebooks and experimental records • Contribute to protocol optimization, troubleshooting, and experimental planning • Present data clearly in lab meetings and communicate progress to the research team Prior experience below is a plus: • Prior experience with human iPSC culture • Experience with CRISPR/Cas9 gene editing • Experience with stem cell differentiation • Experience generating or maintaining brain organoids or other 3D culture systems • Familiarity with aseptic technique, nucleofection, lentiviral production, and sequencing-based validation • Previous experience in an academic, biotech, or translational research laboratory Qualifications Education Bachelor's Degree Related Field of Study required Can this role accept experience in lieu of a degree? Yes Licenses and Credentials Experience Prior laboratory experience, either through academic coursework, internships, or employment 1-2 years preferred Knowledge, Skills and Abilities - Strong theoretical and practical knowledge of laboratory techniques and procedures. - Familiarity with common laboratory equipment and instruments used in research experiments. - Proficiency in data collection, analysis, and scientific software tools. - Excellent attention to detail, organizational skills, and the ability to multitask and prioritize tasks effectively. - Strong problem-solving skills and the ability to troubleshoot technical issues. - Excellent written and verbal communication skills to effectively convey experimental procedures, results, and challenges. Additional Job Details (if applicable) Remote Type Onsite Work Location 185 Cambridge Street Scheduled Weekly Hours 40 Employee Type Regular Work Shift Day (United States of America) Pay Range $21.00 - $29.01/Hourly Grade 5 At Mass General Brigham, we believe in recognizing and rewarding the unique value each team member brings to our organization. Our approach to determining base pay is comprehensive, and any offer extended will take into account your skills, relevant experience if applicable, education, certifications and other essential factors. The base pay information provided offers an estimate based on the minimum job qualifications; however, it does not encompass all elements contributing to your total compensation package. In addition to competitive base pay, we offer comprehensive benefits, career advancement opportunities, differentials, premiums and bonuses as applicable and recognition programs designed to celebrate your contributions and support your professional growth. We invite you to apply, and our Talent Acquisition team will provide an overview of your potential compensation and benefits package. EEO Statement: 1200 The General Hospital Corporation is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment. To ensure reasonable accommodation for individuals protected by Section 503 of the Rehabilitation Act of 1973, the Vietnam Veteran’s Readjustment Act of 1974, and Title I of the Americans with Disabilities Act of 1990, applicants who require accommodation in the job application process may contact Human Resources at View phone number on click.appcast.io. Mass General Brigham Competency Framework At Mass General Brigham, our competency framework defines what effective leadership “looks like” by specifying which behaviors are most critical for successful performance at each job level. The framework is comprised of ten competencies (half People-Focused, half Performance-Focused) and are defined by observable and measurable skills and behaviors that contribute to workplace effectiveness and career success. These competencies are used to evaluate performance, make hiring decisions, identify development needs, mobilize employees across our system, and establish a strong talent pipeline. Combat disease. Hold a hand. Help people. Impact the world. Mass General Brigham is a passionate, welcoming community where minds meet caring hearts. Come be a part of the world’s most powerful force in medicine, where every role is important in changing lives. Are you ready? Our history includes New England hospitals founded over 200 years ago, some of the first and most prestigious hospitals in the world. Built on the legacy of two leading academic medical centers, we’re more than a system—we’re leaders in the practice of medicine. Mass General Brigham is committed to serving the community. We are dedicated to enhancing patient care, teaching and research, and taking a leadership role as an integrated health care system. We recognize that increasing value and continuously improving quality are essential to maintaining excellence.