Research Technologist 1

Research Technologist

Research Technologist in Dr. Jason Brickner's molecular biology lab: Genomes are spatially organized within the nucleus. This spatial organization requires physical interactions between chromatin and subnuclear structures and impacts gene expression. We study how the nuclear pore complex interacts with the genome and how this interaction affects transcriptional regulation and histone modifications. We pursue these questions in the model organism Saccharomyces cerevisiae using a multi-disciplinary approach involving cell biology, molecular genetics, genomics and biochemistry. We are seeking a highly motivated colleague who works well with others who will contribute to these studies and help maintain the lab. This will be a 1-year renewable position based upon performance and funding availability. This position is a FULL-TIME position at 40.0 hours per week and works on-site. The target hiring range for this position will be between $37,062 - $46,124 per year, which is $17.75 - $22.09 per hour. The salary offered will be determined by the applicant's education, experience, knowledge, skills, and abilities, as well as internal equity and alignment with market data.

Specific Responsibilities:

• Support current research directions in the lab, by conducting experiments using biochemistry, molecular biology or cell biology approaches.
• Molecular Biology: DNA, RNA, Protein isolation, purification and expression
• Western blotting
• PCR, RT-PCR
• Plasmid preparation
• Cloning, mapping and sequencing of DNA
• Cell Biology: Yeast and bacterial transformation and culturing
• Sterile technique
• Microscopy: Confocal microscopy and image analysis
• Other (please specify): Solution making
• Lab maintenance
• Chromatography (FPLC)
• Familiarity with R and/or Python for data analysis
• Maintain/organize detailed records of experimental details and data, analyze data, troubleshoot protocols, and recommend alternatives to improve processes, validity, and/or accuracy.
• Support lab maintenance, organization, and operations by preparing key reagents, maintaining equipment, tracking lab supplies and orders, and enforcing lab safety.
• Train students, pilot new directions under guidance, and support collaborations.
• Miscellaneous Performs other duties as assigned.

Minimum Qualifications:

HS diploma or equivalent and some basic science courses is required

Preferred Qualifications:

BA or BS in biology, biochemistry, cell biology, molecular biology or other related biological science discipline. Undergraduate research experience, or prior lab technician experience in these areas.

Preferred Competencies: (Skills, knowledge, and abilities) Basic molecular biology, handling of proteins and nucleic acids, ability to carry out quantitative biochemistry experiments