Research Technician II

Research Technician II The Joag Lab studies tissue-resident memory CD8 T cells (Trm) which stand guard in our airways, lungs, and other mucosal tissues. We want to know how these sentinels change from childhood to adulthood, and how those changes shape our protection against respiratory infections. We are also turning T cells into tools: in fibrotic diseases, where scarred tissue shuts out antibodies and other therapies, we are exploring how T cells can open the door and help treatments reach their targets. The Joag lab was established in 2025, and this position offers the chance to shape lab trajectory and culture from the ground up. General job responsibilities 20% Lab Management: oversee day-to-day operations including ordering, organization, supply management, equipment maintenance, and regulatory compliance to ensure a well-functioning lab. Maintain detailed, updated and accurate records. 25% Animal Colony Management – manage a small-mid sized mouse colony including breeding, genotyping, weaning 55% Experimental Research – design and run flow cytometry experiments. independently perform in vitro and in vivo mouse experiments. Analyze, visualize, and present data to lab. Perform tasks independently and as part of a team effort on larger projects. Preferred Technical Qualifications Multiparameter flow cytometry expertise is a highly valued asset Handling mice, administration of substances to mice via various routes (i.p., intranasal, intra-tracheal route, tail vein), and managing mouse colony Sterile cell culture technique, making buffers, solutions, dilutions Detailed note-taking, protocol writing, and experimental planning Tissue harvesting and enzymatic digestion Desired Experimental skills Injection of mice via RO., I.T., I.N., IP, IV routes PCR, agarose gel electrophoresis In your response, please identify specific examples from your past work, school or other experience that clearly highlight these experiment skills and personality traits. Please only describe your own prior experience. Required Education and Experience Bachelors Degree in biology, microbiology, biochemistry, immunology, molecular biology, or related field. At least one (1) year of experience in a laboratory/research setting and/or experience with molecular biology and biochemistry techniques. Required Credentials N/A. Compensation Range $23.73 - $35.59 per hour Salary Information This compensation range was calculated based on full-time employment (2080 hours worked per calendar year). Offers are determined by multiple factors including equity, skills, experience, and expertise, and may vary within the range provided. Disclaimer for Out of State Applicants This compensation range is specific to Seattle, positions located outside of Seattle may be compensated differently depending on various factors. Benefits Information Seattle Children’s offers a generous benefit package, including medical, dental, and vision plans, 403(b), life insurance, paid time off, tuition reimbursement, and more. Additional details on our benefits can be found on our website About Us Hope. Care. Cure. These three simple words capture what we do at Seattle Children’s – to help every child live the healthiest and most fulfilling life possible. Are you ready to engage with a mission-driven organization that is life-changing to many, and touches the hearts of all? #HOPECARECURE Our founding promise to the community is as valid today as it was over a century ago: we will care for all children in our region, regardless of the families’ ability to pay. Together, we deliver superior patient care, advance new discoveries and treatments through pediatric research, and serve as the pediatric and adolescent, academic medical center for Washington, Alaska, Montana and Idaho – the largest region of any children’s hospital in the country. U.S. News & World Report consistently ranks Seattle Children’s among the nation’s best children’s hospitals. For more than a decade, Seattle Children’s has been nationally recognized in key specialty areas. We are honored to be one of the nation’s very best places to care for children and the top-ranked pediatric hospital in Washington and the Pacific Northwest. As a Magnet designated institution, we recognize the importance of hiring and developing great talent to provide best-in-class care to the patients and families we serve. Our organizational DNA takes form in our core values: Compassion, Excellence, Integrity, Collaboration, Equity and Innovation. Whether it’s delivering frontline care to our patients in a kind and caring manner, practicing the highest standards of quality and safety, or being relentlessly curious as we work towards eradicating childhood diseases, these values are the fabric of our culture and community. The future starts here. Our Commitment Seattle Children’s welcomes people of all experiences, backgrounds, and thoughts as this is what drives our spirit of inquiry and allows us to better connect with our patients and families. 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