Staff Research Associate

Job Description The Huang Lab at UCSF ( investigates the genetics and biology of leukemia through the use of relevant mouse and cell line models. Specific goals include: Determine the biochemical mechanisms governing growth, survival and differentiation of leukemia cells. Identify genes and biochemical pathways that are regulated differentially in leukemia versus normal cells. Measure the growth of distinct populations of normal and leukemia bone marrow cells in vivo and in vitro. Harvest murine bone marrow and embryonic fetal livers for transplantation into irradiated recipients. Transplant genetically modified hematopoietic cells into recipient mice. Perform genetic analysis on mouse tissues. Administer chemotherapeutic agents, radiation, and new experimental treatments to mice. This SRA 1 position is for an individual who will work on leukemia research in mouse models and cell lines. SRA duties will include: Cell/tissue culture Biochemical assays (western blotting, immunoprecipitation, qPCR) DNA and RNA extraction and manipulation PCR and agarose gel electrophoresis Generation of recombinant retroviruses/lentiviruses and transduction of hematopoietic cells Flow cytometry Maintenance of accurate and complete records Organization and maintenance of mouse colonies (including computerized record‑keeping, breeding, monitoring and genotyping) Experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues) Key Responsibilities 20% – Perform organization and maintenance of a large, complex mouse colony (computerized record‑keeping, breeding, monitoring, genotyping) 20% – Perform experimental procedures using laboratory mice (blood sampling, intravenous/intraperitoneal injection, dissection, analysis of harvested tissues) 20% – Perform cell and tissue culture 15% – Perform biochemical assays (western blotting, immunoprecipitation, fractionation, GAP assays) 5% – Perform DNA and/or RNA isolation and manipulation 5% – Perform PCR and agarose gel electrophoresis 5% – Perform generation of recombinant retroviruses or lentiviruses and transduction of hematopoietic cells 5% – Maintenance of accurate, complete and legible records 5% – Perform flow cytometry Qualifications Required Qualifications: BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; and/or an equivalent combination of education and experience Preferred Qualifications: Expertise in breeding and maintaining laboratory mice Molecular biology skills including DNA/RNA extraction and PCR amplification Tissue culture skills including passaging cell lines Biochemistry skills including western blotting About UCSF The University of California, San Francisco (UCSF) is a leading university dedicated to promoting health worldwide through advanced biomedical research, graduate‑level education in the life sciences and health professions, and excellence in patient care. It is the only campus in the 10‑campus UC system dedicated exclusively to the health sciences. UCSF is home to five Nobel laureates who have advanced the understanding of cancer, neurodegenerative diseases, aging and stem cells. UCSF is a diverse community that values professionalism, respect, integrity, diversity, and excellence (PRIDE). We are also committed to equity in all aspects of our workforce and delivery of care, nurturing a welcoming and supportive culture, and engaging diverse ideas for culturally competent education, discovery, and patient care. Equal Employment Opportunity The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law. Salary Information The final salary and offer components are subject to additional approvals based on UC policy. Your placement within the salary range is dependent on a number of factors including your work experience and internal equity within this position classification at UCSF. For positions represented by a labor union, placement within the salary range will be guided by the rules in the collective bargaining agreement. To learn more about the benefits of working at UCSF, including total compensation, please visit #J-18808-Ljbffr University of California, San Francisco