Research Technician II - PSC Core Facility

Research Technician II

The Pluripotent Stem Cell (PSC) Core within the Center for Cellular and Molecular Therapeutics at The Children's Hospital of Philadelphia (CHOP) is seeking a highly motivated Research Technician II. This position provides hands-on support for core services involving human pluripotent stem cells, including iPSC culture, differentiation workflows, and CRISPR-based gene editing, in a fast-paced, highly collaborative environment. The successful candidate will be exceptionally detail-oriented, organized, and committed to thorough documentation to support reproducibility and core operations.

This role will focus on routine maintenance and expansion of PSC/iPSC lines, execution of differentiation workflows as needed, and participation in CRISPR gene editing and downstream screening/validation support. Strong note-taking, sample tracking, and organizational skills are essential.

What You Will Do:

• Maintain and expand PSC/iPSC cultures (routine culture, passaging, cryopreservation, thawing, QC support)
• Perform differentiation workflows as needed (neural lineage experience is a plus)
• Execute CRISPR gene editingrelated tasks (e.g., preparation, delivery support, and coordination of downstream clone screening/validation workflows as appropriate for the core)
• Follow established SOPs and contribute to protocol optimization when appropriate
• Prepare reagents, maintain inventory, and support ordering/supply management
• Maintain shared lab/core spaces, equipment logs, and general lab organization
• Communicate effectively with core staff, investigators, and trainees; support core users as needed
• Maintain excellent documentation: clear experimental notes, sample labeling and tracking, data organization, and accurate records in electronic and/or paper notebooks (per core requirements)
• Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
• Performs research procedures on experimental samples as directed
• Records and checks experiments/ results for completeness and accuracy
• Performs calculations and maintains appropriate log and lab notebooks
• Performs tasks that require familiarity with fundamental techniques and lab equipment
• May assist with planning specific research protocols and procedures
• Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
• Assists in care and maintenance of lab equipment
• May coordinate schedules of tests and procedures
• Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
• Records results, organizes data and maintains lab notebook in an organized manner
• Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
• Conducts literary searches as directed

Education Qualifications:

• High School Diploma / GED - Required
• Associate's Degree - Preferred
• Bachelor's Degree - Preferred

Experience Qualifications:

• At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Required
• At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Preferred

Skills and Abilities:

• Intermediate understanding of lab procedures, protocols, and equipment.
• Basic exposure to lab specific experiments / techniques and scientific analysis
• Excellent verbal and written communications skills
• Excellent organizational skills
• Solid critical thinking / problem-solving skills
• Solid analytical skills

To carry out its mission, CHOP is committed to supporting the health of our patients, families, workforce, and global community. As a condition of employment, CHOP employees who work in patient care buildings or who have patient facing responsibilities must receive an annual influenza vaccine.

Salary Range: $43,900.00 - $54,800.00 Annually

At CHOP, we are committed to fair and transparent pay practices. Factors such as skills and experience could result in an offer above the salary range noted in this job posting. Click here for more information regarding CHOP's Compensation and Benefits.

Children's Hospital of Philadelphia