Research Assistant

The Sidarala Laboratory is seeking a highly motivated Research Assistant to join a dynamic research program focused on understanding how mitochondrial dynamics regulates pancreatic β-cell function, aging, and diabetes. The successful candidate will contribute to projects investigating the cellular and molecular mechanisms underlying type 2 diabetes using cutting-edge molecular biology and imaging approaches. This position is ideal for individuals interested in gaining extensive research experience before pursuing graduate, medical, or professional school, or for candidates seeking a career in biomedical research. Outstanding candidates will have opportunities to co-author peer-reviewed publications and present research at regional and national scientific meetings. Primary Responsibilities The Research Assistant will: Manage and maintain mouse colonies, including colony organization, record keeping, and health monitoring Establish breeding schemes and genotyping workflows to generate experimental mouse cohorts Assist with and perform in vivo studies, including animal handling, injections, metabolic phenotyping, glucose and insulin tolerance tests, blood and tissue collection, and other physiological assessments (training provided as needed) Perform pancreatic islet isolations from mice (training provided as needed) Perform molecular biology techniques including DNA / RNA extraction, PCR , qPCR, cloning, and genotyping Conduct biochemical assays including Western blotting, ELISA , insulin secretion assays, and enzyme activity measurements Prepare laboratory reagents and solutions, maintain laboratory equipment, manage inventory, and ensure compliance with laboratory safety procedures Organize, analyze, and maintain accurate records of experimental data using electronic laboratory notebooks Participate in laboratory meetings and contribute to manuscript preparation and grant applications as appropriate Research Areas Projects in the laboratory focus on: Pancreatic islet biology and β-cell physiology Mitochondrial dynamics, cellular metabolism and bioenergetics Aging and age-associated metabolic diseases Translational studies using mouse and human β-cell models Training Opportunities The Research Assistant will receive training in modern techniques including: Pancreatic islet isolation and functional assays Live-cell and super-resolution confocal imaging Metabolic phenotyping Molecular and cellular biology Experimental design and statistical analysis Scientific writing and presentation skills Required Qualifications Bachelor’s degree in Biology, Biochemistry, Molecular Biology, Biomedical Sciences, or a closely related field Strong organizational and communication skills Ability to work both independently and collaboratively Excellent attention to detail and commitment to maintaining accurate research records; Willingness to learn new laboratory techniques Preferred Qualifications Experience working with laboratory mice, including animal handling and husbandry Experience managing transgenic or knockout mouse colonies, including breeding strategies and colony record keeping Familiarity with mouse genotyping techniques, including DNA extraction and PCR Experience with in vivo procedures such as injections, blood collection, metabolic phenotyping, or tissue harvesting Experience with pancreatic islet isolation or other tissue dissection techniques Experience with mammalian cell culture and molecular biology techniques, including PCR , qPCR, Western blotting, or cloning Familiarity with immunofluorescence microscopy or confocal microscopy Location Location MURC - Marshall University Research Corporation Salary Range Salary Range Salary will be commensurate with experience and qualifications. Required Qualifications Education Bachelor’s degree in Biology, Biochemistry, Molecular Biology, Biomedical Sciences, or a closely related field Experience Strong organizational and communication skills Ability to work both independently and collaboratively Excellent attention to detail and commitment to maintaining accurate research records; Willingness to learn new laboratory techniques Preferred Qualifications Education Experience Experience with one or more of the following is desirable but not required: Experience working with laboratory mice, including animal handling and husbandry Experience managing transgenic or knockout mouse colonies, including breeding strategies and colony record keeping Familiarity with mouse genotyping techniques, including DNA extraction and PCR Experience with in vivo procedures such as injections, blood collection, metabolic phenotyping, or tissue harvesting Experience with pancreatic islet isolation or other tissue dissection techniques Experience with mammalian cell culture and molecular biology techniques, including PCR , qPCR, Western blotting, or cloning Familiarity with immunofluorescence microscopy or confocal microscopy Posting Number Posting Number MR0887 Open Date Open Date 07/30/2026 Close Date Open Until Filled Yes Special Instructions Summary Supplemental Questions Required fields are indicated with an asterisk (*). * Do you have a Bachelor's degree in Biology, Biochemistry, Molecular Biology, Biomedical Sciences, or a closely relatedfield? Yes No #J-18808-Ljbffr Marshall University