Postdoctoral Researcher

The Department of Veterinary Science at the University of Kentucky is seeking a highly motivated, detail-oriented, and experienced Research Analyst / Genomics Research Specialist to support research in microbial whole-genome sequencing,RNAsequencing, antimicrobial resistance, bacteriophage biology, host-pathogen interactions, and One Health infectious diseases. This position offers an opportunity to work at the interface of microbial genomics, transcriptomics, infectious disease research, antimicrobial resistance surveillance, bacteriophage-based interventions, and host-pathogen interaction studies. The successful candidate will contribute to sequencing-based projects involving bacterial pathogens, bacteriophages, host cells, environmental specimens, organoid-derived samples, and animal-associated samples. We are seeking an experienced sequencing specialist with strong hands-on library preparation forRNAandDNAsequencing, molecular biology and next-generation sequencing experience, demonstrated troubleshooting ability, excellent organizational skills, and the ability to contribute to experimental design, sequencing strategy, protocol optimization, quality-control interpretation, and workflow development. For more information, please contact us at View email address on click.appcast.io Key Responsibilities Experimental Design and Sequencing Strategy Assist with experimental design for microbialWGS,RNA-seq, transcriptomic, metagenomic, metatranscriptomic, bacteriophage, and host-pathogen interaction studies. Help determine appropriate sequencing approaches based on project goals, sample type, organism, genome size,RNAquality, read length, sequencing depth, and downstream analysis needs. Contribute to decisions regarding Illumina short-read sequencing, Oxford Nanopore sequencing, PacBio long-read sequencing, and hybrid sequencing strategies. Assist with planning biological replication, sample batching, barcode assignment, library pooling, run design, and quality-control checkpoints. Develop troubleshooting plans for low nucleic acid recovery,RNAdegradation, contamination, failed libraries, low sequencing output, poor read quality, barcode imbalance, and insufficient sequencing depth. Work with the principal investigator, trainees, bioinformaticians, sequencing facilities, and collaborators to ensure that sequencing workflows support genome assembly, transcriptomics, antimicrobial resistance analysis, virulence analysis, sequence typing, phylogenetics, and public database submission. Contribute to the development, validation, scaling, and refinement of sequencing workflows and standard operating procedures. Participate in evaluation and implementation of new sequencing technologies, library-preparation methods, quality-control approaches, and workflow improvements. DNA/RNAExtraction, Library Preparation, and Quality Control Perform high-quality genomicDNAand totalRNAextraction from bacterial isolates, bacteriophages, environmental specimens, animal-associated samples, host cells, organoid-derived samples, and host-pathogen interaction experiments. OptimizeDNAandRNAextraction protocols for difficult organisms, low-biomass specimens, intracellular pathogens, bacteriophages, and complex biological samples. Perform high-molecular-weightDNAextraction for long-read sequencing and supportRNAstabilization and preservation for transcriptomic applications. Prepare sequencing libraries for bacterialWGS,RNA-seq, host-response studies, bacteriophage genomics, amplicon sequencing, and other genomic applications. Support Illumina library preparation, Oxford Nanopore ligation/rapid/native barcoding/cDNA workflows, and PacBio SMRTbell or related long-read sequencing workflows. Support bacterial rRNA depletion, strand-specificRNA-seq workflows, and transcriptomic library preparation.li> AssessDNA/RNAquantity, purity, integrity, molecular weight, and library quality using Qubit, NanoDrop, Bioanalyzer, TapeStation, Fragment Analyzer, gel electrophoresis, or comparable platforms. Interpret quality-control results and recommend whether samples or libraries are suitable for sequencing. Troubleshoot low nucleic acid recovery, poorDNAquality,RNAdegradation, low library yield, adapter-dimer formation, contamination, poor read quality, and suboptimal fragment-size distribution. Sequencing Workflow Support and Data Organization Prepare sample sheets, barcoding plans, run manifests, metadata files, and sequencing submission documentation. Coordinate sequencing runs and sample submissions to internal core facilities, external sequencing centers, and collaborating laboratories. Monitor library yield, sequencing run performance, read output, read quality, sequencing depth, and basic quality metrics. Assist with base-calling, demultiplexing coordination, raw data organization, file transfer, and documentation. Support hybrid genome assembly projects combining Illumina short-read data with Oxford Nanopore or PacBio long-read data. Collaborate with bioinformatics personnel to ensure that sequencing data and metadata are appropriately formatted for genome assembly, annotation,AMRanalysis, sequence typing, virulence analysis, phylogenetics, transcriptomics, and repository submission. Maintain accurate sample inventories, freezer records, sequencing logs, reagent records, quality-control records, and run documentation. Assist with preparation and submission of sequencing data to repositories such asNCBISRA, GenBank, BioProject, and BioSample. Support preparation of sequencing-related summaries, technical reports, manuscript methods, grant progress reports, and workflow documentation. Molecular Biology, Biosafety, and Team Collaboration PerformPCR, qPCR, reverse-transcriptionPCR, gel electrophoresis, nucleic acid cleanup, size selection, and related molecular biology procedures. Operate and maintain commonly used laboratory equipment, including centrifuges, electrophoresis systems, spectrophotometers, fluorometers, thermocyclers, qPCR instruments, and nucleic acid quality-control instruments. Follow institutional biosafety, chemical safety, data-management, and laboratory quality-control procedures, includingBSL-2 practices when applicable. Participate in laboratory meetings and provide regular updates on sample processing, library preparation, sequencing progress, quality-control results, experimental design issues, and technical challenges. Communicate technical findings clearly to laboratory members, collaborators, sequencing facilities, and bioinformatics personnel. Assist trainees and laboratory members with sequencing-related protocols, sample tracking, quality-control procedures, documentation, and good laboratory practices. Contribute to a collaborative, organized, respectful, and productive research environment. Required Qualifications Applicants should have a Master’s degree in genomics, molecular biology, microbiology, genetics, biotechnology, bioinformatics, or a closely related sequencing-focused field. A PhD is preferred, particularly for candidates with experience in microbial genomics,RNA-seq, long-read sequencing, bacteriophage genomics, host-pathogen interactions, or sequencing-based infectious disease research. Exceptional candidates with a Bachelor’s degree and extensive relevant sequencing experience may also be considered. The successful candidate should have strong hands-on experience with microbial whole-genome sequencing,RNA-seq, sequencing library preparation, nucleic acid quality control, and molecular biology techniques, includingDNA/RNAextraction,PCR, qPCR, RT-PCR, gel electrophoresis, nucleic acid cleanup, quantification, and library quality assessment. Experience with Illumina, Oxford Nanopore, and/or PacBio workflows is highly desirable, including short-read sequencing, long-read sequencing, high-molecular-weightDNAextraction, bacterialRNAextraction, rRNA depletion, strand-specificRNA-seq, hybrid genome sequencing, amplicon sequencing, or relatedNGSapplications. Applicants should be able to contribute to the design, optimization, troubleshooting, validation, and documentation of sequencing-based studies and SOPs. Relevant experience includes sequencing platform selection, sample-preparation strategy, library-preparation planning, sequencing-depth considerations, sample batching, barcoding, metadata management, contamination prevention, and troubleshooting of low-yield samples, failed libraries, or sequencing-quality issues. Experience working with bacterial pathogens, bacteriophages, intracellular organisms, host cells, organoids, environmental specimens, animal-associated samples, orBSL-2 workflows is preferred. Familiarity with microbial genomics workflows, including genome assembly, annotation, antimicrobial resistance detection, virulence analysis, sequence typing, phylogenetics, outbreak investigation, public database submission, and One Health surveillance, is highly desirable. The successful candidate should have strong attention to detail, problem-solving ability, organizational skills, independence, teamwork, and written and verbal communication skills. The candidate should be able to maintain complete laboratory records, manage multiple sequencing projects, communicate clearly with laboratory members and collaborators, and support preparation of sample sheets, run plans, metadata files, reports, manuscripts, grant-related summaries, SOPs, and public sequence-database submissions. #J-18808-Ljbffr University of Kentucky