Senior Associate Scientist, Cell Therapy

• Perform primary immune cell isolation and culture, as well as cancer cell line culture.
• Perform mRNA-based CAR T cell engineering using electroporation and tLNP
• Lead functional characterization of CAR T cells with immunoassays
• Data analysis and thorough documentation of work in the Company’s ELN system
• Present results at team meetings, in other group settings, and at external scientific conferences
• Work cross-functionally as part of internal multi-disciplinary project teams
• Maintaining a safe and clean laboratory environment following all precautions required for a BSL2/BSL2+ setting.

• Senior Associate Scientist: B.S./M.S. in immunology, biology, oncology or related discipline with BS 6+ years or MS 4+ years of relevant industry experience
• Strong hands-on experience with primary human immune cell and cancer cell line culture, T cell transfection via mRNA electroporation, cell immunophenotyping using multicolor flow cytometry, cytotoxicity assays, multiplex ELISA, live-cell analysis using incucyte system
• Proficiency in operating instruments like BD Fortessa flow cytometer, Lonza 4D-Nucleofector, Meso Scale Discovery plate reader, xCELLigence RTCA
• Working knowledge of CAR T cell therapy discovery
• Ability to follow defined procedures and protocols, and strong attention to detail
• Proficiency with data analysis software such as FlowJo, GraphPad Prism, etc
• Willingness to learn new research methods and a desire for continued learning and development
• Excellent organization and record-keeping skills
• Collaborative, flexible, and team-oriented mindset, with the ability to contribute effectively within a dynamic research environment

• Direct experiences with mRNA/tLNP CAR T engineering and characterization
• Knowledge of building high-throughput T cell engineering and characterization workflow
• Track record of assay development and optimization in an in vitro pharmacology setting
• Knowledge of molecular biology techniques, including mRNA IVT, real-time PCR, and ddPCR
• Experience in immunophenotyping tumor-infiltrating T cells in CDX/PDX mouse model

At PharmaEssentia, our goal is to treat as many people with cancer as possible. That means challenging the status quo with better science that leads to better lives. By joining our team, you will not only expand your own possibilities, but you will contribute to expanding options for people with cancer.