Research Associate II

Research Associate — Stem Cell Biology, Genome Engineering, and Genomics

The Maehr Lab at UMass Chan Medical School invites applications for a Research Associate position supporting an NIH-funded program focused on engineering stem cell–derived islets for immune tolerance.

Our laboratory combines human pluripotent stem cell differentiation, CRISPR-based genome and epigenome engineering, next-generation sequencing, single-cell genomics, and immunology to develop stem cell–derived islets that resist autoimmune and allogeneic immune responses while preserving beta-cell identity and function.

The Research Associate will support projects involving human pluripotent stem cell culture and differentiation, molecular cloning, gene targeting, immune assays, and genomic profiling. The successful candidate will work with experimental and computational scientists to generate and characterize engineered stem cell lines and assess their differentiation, function, molecular state, and immune compatibility.

Applicants should have a bachelor's or master's degree in biology, molecular biology, cell biology, genetics, genomics, bioengineering, immunology, or a related field.

Experience in one or more of the following areas is preferred:

• Human pluripotent stem cell culture and directed differentiation.
• Molecular cloning and CRISPR-based gene targeting.
• RNA-seq, ATAC-seq, ChIP-seq, or single-cell omics.
• Flow cytometry, imaging, or immune-cell assays.
• Pancreatic beta-cell or islet biology.

Prior experience in every area is not required. Candidates with strong experience in one relevant area and an interest in learning complementary approaches are encouraged to apply.

Responsibilities

Essential functions include:

• Perform laboratory experiments and may be responsible for a single research project
• Utilize all software relevant to experiment and equipment used
• Interpret data, form conclusions and decide on next steps with a degree of independence while under the direction of the PI or designee
• Maintain accurate records, may modify protocols with the approval of the PI or designee
• Review literature, publications and grants relevant to assigned projects
• Record results according to established lab, sponsor & university protocol
• Assist with performing data analysis and/or mathematical computations
• Maintain a detailed lab notebook according to lab practice
• Present findings at lab/group meetings
• Monitor laboratory supply levels and order as noted
• May assist with laboratory equipment service contracts and monitor maintenance on laboratory equipment
• Prepare and label buffers, media, solutions, etc.
• Assist with and comply with all safety regulation and infection control standards
• Train on IACUC, IRB, EHS, Radiation safety protocols
• Comply with all applicable state, federal university, and sponsor policies and agreements
• Provide support with identifying problems and challenges, propose resolutions, implement appropriate solutions
• May assist with the training of new lab personnel
• Provide support with drafting standard operation procedures in compliance to EHS regulation specific for the lab
• May assist with drafting manuscripts sections
• Perform other duties as required

Qualifications

Required qualifications include:

• Bachelor's degree in Biological or Natural Science and 18 months of relevant research experience or a related Master's degree.
• Knowledge of Microsoft Office products
• Ability to analyze complex visual data in a quantitative and objective manner
• Ability to work in a team environment

Additional Information

The Maehr Lab is part of the Program in Molecular Medicine and the Diabetes Center of Excellence at UMass Chan Medical School. The laboratory offers a highly collaborative environment spanning stem cell biology, immunology, functional genomics, epigenomics, computational biology, and regenerative medicine.