Life Science Research Professional 2, General Surgery

The Cepika Lab, in the Division of General Surgery, Department of Surgery at Stanford University School of Medicine, studies human immune tolerance. Using cellular immunology, genomics and gene engineering, we aim to understand (1) the role of human thymic regulatory T cells (Tregs) and inducible type 1 regulatory T (Tr1) cells in resistance to anti-tumor immunity and cancer immunotherapy, and (2) the interplay between innate and adaptive immunity in the pathogenesis of human inflammatory bowel disease (IBD). The lab is affiliated with the Stanford Cancer Institute, Stanford Bio-X, Center for Definitive and Curative Medicine, and the Maternal & Child Health Research Institute, and works closely with clinical teams and shared research facilities across the School of Medicine. We are seeking a Life Science Research Professional 2 to lead the single-cell genomics and flow cytometry portion of both research programs. The successful candidate will independently design, execute, analyze and troubleshoot experiments on primary human patient samples, perform tissue culture, develop new assays and protocols where none exist, contribute substantively to publications and presentations, serve as the laboratory's technical resource and trainer for cytometry and single-cell methods, mentor students and interns, and help oversee day-to-day laboratory operations. Desired Qualifications: Experience in human immunology and translational research using primary human patient samples. Desired experience and skills include: Single-cell RNA-sequencing library preparation and downstream bioinformatic analysis in R, including quality control, normalization, integration and batch correction, clustering, cell type annotation, differential expression and pseudotime/trajectory inference Command-line/UNIX proficiency and familiarity with sequence alignment and genomics tools (e.g. STAR, BWA) High-parameter flow cytometry panel design, staining, acquisition and analysis (FlowJo), including resolution of rare cell populations Independent operation, daily QC, maintenance, troubleshooting, scheduling and user training of a cell sorter (BD FACSAria II/III or equivalent) Isolation, processing, cryopreservation and thawing of human PBMC and tissue-derived cells, including intestinal biopsies; primary human T cell and antigen-presenting cell culture, stimulation, and in vitro differentiation, suppression and killing assays Independent development and optimization of novel assays and protocols Nucleic acid isolation, quantitation and quality control, cDNA synthesis, RT-qPCR and primer design ELISA and multiplex cytokine assays; plasmid design and molecular cloning (site-directed mutagenesis, PCR, transformation, miniprep) Familiarity with lentiviral vector production and gene editing of primary human cells is desirable Statistical analysis and scientific graphing (R, GraphPad Prism) Experience with IRB-approved human subjects research samples Experience training and supervising junior researchers Experience managing laboratory operations, inventory and procurement; Proficiency with the Microsoft Office package Excellent written and oral scientific communication and a demonstrated ability to work effectively in a collaborative, multidisciplinary environment #J-18808-Ljbffr Stanfordlivetickets