Scientist I/Scientist II, tLNP Formulation and Delivery

Profluent seeks a foundational scientist to design, formulate, and characterize targeted lipid nanoparticles for next-generation genetic medicines. The role spans formulation optimization, bioconjugation, analytical characterization, and in vitro/in vivo validation to achieve extrahepatic tissue targeting.

Responsibilities

• Design and execute tLNP formulations with nucleic acid payloads by systematically varying lipid ratios and helper components
• Perform surface modifications and bioconjugation chemistries to attach targeting moieties to LNP surfaces
• Execute LNP analytics including particle characterization, encapsulation efficiency, and degree of labeling quantification
• Establish mammalian cell culture models and develop in vitro assays to evaluate targeting, uptake, and functional cargo translation
• Design in vivo biodistribution and pharmacokinetic studies; analyze datasets to establish structure-activity relationships

Requirements

• Ph.D. in Biomedical Engineering, Chemical Engineering, Pharmaceutical Sciences, or related field
• 0–2 years (Scientist I) or 2–4 years (Scientist II) of relevant experience
• Hands-on experience with LNP assembly platforms and nanoparticle surface bioconjugation techniques
• Demonstrated ability to modify nanoparticle biodistribution through lipid composition and structural alterations
• Proficiency in mammalian cell culture and quantitative cell-based assays (Flow Cytometry, reporter assays, ELISAs)
• Understanding of systemic nanoparticle distribution, protein corona, vascular barriers, and in vivo study design