Research Technician - Chouchani Lab

Research Technician Position

We have an opportunity for a Research Technician to join the Chouchani lab at the Dana-Farber Cancer Institute. We tend to think of metabolites as simple building blocks and fuel for biological processes. But many metabolites appear to play crucial roles in regulating living systems. This concept of metabolite signaling remains poorly understood at the biochemical level, and we still do not understand how most metabolite signals work. Edward Chouchani's lab is developing new technologies to better understand the rules of the language of core metabolite signals. The hope is that by understanding how nature uses metabolism to regulate biological systems, we will have new opportunities for treating human disease.

About the Research Technician role:

The Edward Chouchani Lab has an exciting new opportunity for a research technician I position. The Chouchani Lab is located at the Dana-Farber Cancer Institute and is affiliated with Harvard Medical School. This position will assist scientists in performing scientific research of varying complexity. Duties include working with laboratory mice and conducting basic molecular biology and cell biology techniques. Basic procedures include but are not limited to pipetting, cell and tissue culture, handling of small laboratory animals, etc. There will be continuous learning opportunities, and the position will be a good steppingstone for those interested in biomedical research.

What we provide:

• Ample opportunities and support for upskilling. Coworkers that assist in learning new techniques/skills.
• Mentorship opportunities.
• Opportunities for professional growth and development, as well as opportunities to make scientific contributions.
• A competitive compensation package with comprehensive health and welfare benefits.

What you'll do:

• Assisting postdocs and students with cell-based assays, which will involve maintenance of cell lines and perform routine molecular and cellular biology experiments (e.g., qPCR, western blot, sequencing, plasmid purification and bacterial transformation).
• Assisting with biochemical techniques such as protein purification and quantification, enzyme assays and metabolite measurements.
• Assisting with metabolomic and proteomic sample preparation for mass spectrometry analysis.
• Procurement tasks such as ordering, checking off deliveries, distributing packages and maintaining stocks of consumables.
• General lab management, organization and day-to-day operations.

What you bring:

• Bachelors or masters degree in biology, biotechnology, or a related field.
• Hands-on laboratory experience in molecular and/or cell biology.
• Previous experience working in a medical or scientific research setting is preferred.
• Must be self-motivated and an independent worker with excellent communication skills (written and verbal).
• Familiarity with standard molecular biology techniques (e.g., PCR, western blot, cloning)
• Experience with genetic sequencing and basic data analysis.
• Prior experience with T cells or other immune cells (e.g., primary T cells from PBMCs or other systems)
• Familiarity with standard molecular biology techniques (e.g., PCR, western blot, cloning)
• Experience with genetic sequencing and basic data analysis.
• Prior experience with CRISPR screening is preferred but not strictly required.
• Prior experience with mice handling and mice work is good to have but not required.

Physical Requirements

Remaining in a normal seated or standing position for extended periods of time; reaching and grasping by extending hand(s) or arm(s); dexterity to manipulate objects with fingers, for example using a keyboard; communication skills using the spoken word; ability to see and hear within normal parameters; ability to move about workspace. The position requires mobility, including the ability to move materials weighing up to several pounds (such as a laptop computer or tablet). Work may involve possible exposure to malodorous vapors, low dose radiation, contamination by toxic chemicals and acids and presence of carcinogenic substances or other hazardous materials.

Persons with disabilities may be able to perform the essential duties of this position with reasonable accommodation. Requests for reasonable accommodation will be evaluated on an individual basis.

Compensation and Benefits

Our employees are compensated from a total rewards perspective in many ways for their contributions to our mission, including competitive pay, exceptional health benefits, retirement plans, time off, and a range of recognition and wellness programs. Visit our Benefits at HHMI site to learn more.

Hiring Pay Range

$18.40 - $23.03

The posted range reflects HHMI's good faith estimate of the anticipated hiring salary range for this role at the time of posting. Actual hiring compensation is determined by a candidate's qualifications, experience, and internal equity.

HHMI is an Equal Opportunity Employer

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