Scientist l, Cell Line Development (based fully onsite)

Responsibilities Execute CHO cell line development and characterization for GMP biotherapeutics: transfection, selection, high-throughput clone screening, and culture scale-up (multi-well → shake flasks → small-scale stirred tanks). Perform random/targeted integration approaches (transposase-, recombinase-mediated). Conduct molecular/genetic characterization: DNA/RNA extraction; PCR, qPCR/RT-qPCR, ddPCR, sequencing; support vector design/construction and sequence confirmation. Generate monoclonal CHO cell lines via single-cell cloning; cryopreserve research cell banks and maintain inventory. Assess productivity/stability using fed-batch and parallel cultures on manual/automated platforms. Operate instrumentation (Vi-CELL counters, Octet label-free binding platforms). Develop/optimize methods and support assay qualification/troubleshooting; improve throughput, data quality, and workflow. Maintain ELN records; author/revise protocols; train team members; present updates and collaborate cross-functionally. Follow safety, code of conduct, and GxP compliance. Qualifications BS with 5+ years (or MS with 2+ years) in biologics discovery/development or related field. Aseptic technique; hands-on mammalian (CHO) cell culture; manage multiple projects. Nucleic acid extraction; PCR/qPCR and/or ddPCR. Experience developing stably transfected CHO lines for monoclonal/antibody-like molecules (transfection, selection, minipools, clone isolation/screening, scale-up). Experience with transposase/recombinase technologies. Cryopreservation and research cell bank creation (viability/recovery; inventory record-keeping). Recombinant protein titer quantification (e.g., Octet/biolayer interferometry or equivalent). Plate-based clone screening (96-well, 24‑deep‑well; mapping/sampling; outlier/plate-effect review). Clone productivity/growth/stability assessment (batch/fed‑batch; ranking on manual/automated platforms). Independent experiment design/execution/analysis/troubleshooting; strong mammalian & molecular biology understanding; attention to detail; strong written/verbal communication. Benefits Comprehensive benefits including PTO, medical/dental/vision insurance, and 401(k) for eligible employees; eligible for short-term incentive programs. #J-18808-Ljbffr Scorpion Therapeutics