Scientist I (Gene Therapy/ Cell Cloning)

Job Description

Job Description

Our client, a world leader in life sciences and biotechnology, is looking for a “Scientist I” based out of San Diego, CA.

Job Duration: Long Term Contract (Possibility Of Extension)

Pay Rate : $46.43/hr on W2

Company Benefits: Medical, Dental, Vision, Paid Sick leave, 401K

The Scientist I will be a vital contributor to our non-viral cell and gene therapy delivery platform, primarily focused on R&D for innovative nucleic acid payload technologies. This role involves evaluating novel mRNA and DNA designs using a range of molecular biology techniques, including plasmid cloning, in vitro validation, and the production of in vitro-transcribed mRNA. To verify successful expression, the scientist will utilize assays such as Western blot, qRT-PCR, and luciferase reporter assays.

Job Responsibilities:

· Design and clone expression vectors and IVT donor plasmids to support research initiatives.

· Execute DNA and mRNA transfections in mouse and human cell lines.

· Assess nucleic acid expression through molecular methods like qPCR, reporter assays and Western blotting.

· Maintenance of detailed electronic laboratory notebooks (ELN) and preparation of summary slides

Required Qualifications:

• Bachelor's degree with 5+ years or a Master's degree with 2+ years of experience in molecular biology, pharmaceutical sciences, or a related field.
  
• Molecular Biology: Required proficiency in advanced cloning (Gibson assembly, Golden Gate, site-directed mutagenesis) and IVT.
  
• Functional Assays: Hands-on expertise in measuring transgene delivery success via reporter assays and qRT-PCR.
  
• Cell Culture: Preferred experience in genetic manipulation of cell lines and primary cells (e.g., T cells, monocytes, hepatocytes).
  
• Desired proficiency in Snapgene, Prism, and MS Office.
  

If interested, please send us your updated resume at

View email address on ziprecruiter.com/View email address on ziprecruiter.com