USA - Research Associate - I - USD

Job Responsibilities:

Maintain healthy eukaryotic cell cultures, including insect (Sf9, Sf21, High Five) and/or mammalian expression lines. Generate, amplify, and quality-control baculovirus stocks, including centralized storage and record management. Execute multi-liter scale recombinant protein expression experiments, including infection, culture monitoring, and cell harvest. Perform small-scale, plate-based expression and purification profiling to assess expression levels, solubility, and purification behavior. Maintain accurate and timely records of experimental data, cell line usage, and virus stock inventories. Operate and maintain basic laboratory instrumentation and contribute to general lab support activities as needed. Collaborate closely with team members to optimize expression workflows and troubleshoot technical issues. Skills:

Experience with the Bac-to-Bac baculovirus expression system, including donor plasmid preparation, bacmid generation, and virus amplification. Familiarity with plate-based expression and purification approaches (e.g., 24–96 well plates and rapid IMAC and other affinity-based screens). Experience conducting multi-liter expression campaigns and associated downstream sample handling. Strong analytical, organizational, and documentation skills. Ability to work independently and in cross-functional teams. Education/Experience:

BS degree in Molecular Biology, Cell Biology, Biochemistry, or a related scientific discipline. Minimum of 3 years of hands-on laboratory experience, including work with insect cell culture (e.g., Sf9, Sf21, Hi5) and/or mammalian cell culture systems. Demonstrated experience in aseptic technique and routine maintenance of suspension cultures.