Research Assistant - 140190

Payroll Title: SRA 1 Department: UROLOGY Hiring Pay Scale: $28.69 / Hour Worksite: Moores Cancer Center Appointment Type: Career Appointment Percent: 100% Union: RX Contract Total Openings: 1 Work Schedule: Days, 8-Hour Shifts, Monday-Friday Description Under supervision, assist with experiments, make observations, and record experimental data related to research projects. Use molecular biological techniques to assist in the development of plasmid and viral based gene transfer vectors. Assist with animal studies including breeding transgenic mice, injections, maintaining and screening transgenic mouse colony, in vivo fluorescent and bioluminescent imaging, necropsy, tissue trimming and histology. Prepare tissue cultures of primary lymphocytes and propagation of tumor cell lines. Extract RNA, reverse transcribe cDNA, perform RT-PCR, mini preps of plasmids and analysis by gel. Perform basic cellular immunology tests to assess immunity such as FACS and CFSE proliferative assays, and automatic cell counting. Media and buffer preparation. Maintain lab records, and order supplies as needed. Minimum Qualifications Theoretical knowledge of biochemistry, molecular biology, immunology and genetics, including relevant laboratory techniques, acquired through a Bachelor’s degree in biology or related field; and/or equivalent combination of education and laboratory experience. Experience assisting with tissue culture including freezing, thawing, counting cells and splitting cultures. Experience with data analysis using Excel and Prism software. Experience working with basic aseptic and tissue culture techniques. Ability to work with human tissues; tissue trimming, histology analysis, and knowledge in necropsy. Ability to work with cell culture, media preparation, and DNA electrophoresis. Willingness to follow step-wise experimental protocols. Ability to use basic laboratory equipment including centrifuges, spectrophotometers, balances, pH meters, and electrophoresis equipment. Willingness to become familiar with proper handling of chemical and biohazard materials. Willingness to adhere to established UCSD guidelines for safety and environmental issues. Familiarity with computer software to analyze data, store results and compute raw data. Willingness to maintain confidentiality. Willingness to learn organizational skills as they relate to maintain lab equipment and supplies. Ability to perform basic PCR and ELISA techniques. Willingness to work with other staff members, students, faculty etc. Ability to work with a variety of chemicals and biological materials while following appropriate safety procedures, including working with isoflurane in a fume hood and preparing solutions containing ammonium thiocyanate. Preferred Qualifications Experience using patient databases, such as EPIC. Experience with caring for and handling small lab animals (mice) and in administering medications and anesthesia to perform phlebotomy and minor surgical procedures on animals. Experience euthanizing small laboratory animals. Familiarity with the writing of Animal Subjects protocols and with the surgical procedures. Willingness and ability to work with rodents and/or other lab animals and/or infected samples. Theoretical knowledge of normal and malignant hematopoietic stem and progenitor cell biology sufficient to aid in the design and interpretation of experiments on those topics. Theoretical knowledge of microbiology, cellular, molecular and prostate cancer biology. Knowledge of current prostate cancer disease models and understanding of clinical features of prostate cancer and treatments. Special Conditions Must be able to work various hours and locations based on business needs. Employment is subject to a criminal background check. Pay Transparency Act Annual Full Pay Range: $59,905 - $71,451 (will be prorated if the appointment percentage is less than 100%) Hourly Equivalent: $28.69 - $34.22 Factors in determining the appropriate compensation for a role include experience, skills, knowledge, abilities, education, licensure and certifications, and other business and organizational needs. The Hiring Pay Scale referenced in the job posting is the budgeted salary or hourly range that the University reasonably expects to pay for this position. The Annual Full Pay Range may be broader than what the University anticipates to pay for this position, based on internal equity, budget, and collective bargaining agreements (when applicable). Health and Safety Requirements If employed by the University of California, you will be required to comply with our Policy on Vaccination Programs, which may be amended or revised from time to time. Federal, state, or local public health directives may impose additional requirements. If applicable, life-support certifications (BLS, NRP, ACLS, etc.) must include hands-on practice and in-person skills assessment; online-only certification is not acceptable. Misconduct Disclosure Requirement As a condition of employment, the final candidate who accepts an offer of employment will be required to disclose if they have been subject to any final administrative or judicial decisions within the last seven years determining that they committed any misconduct; or have filed an appeal of a finding of substantiated misconduct with a previous employer. \"Misconduct\" means any violation of the policies governing employee conduct at the applicant’s previous place of employment, including, but not limited to, violations of policies prohibiting sexual harassment, sexual assault, or other forms of harassment, or discrimination, as defined by the employer. For reference, below are UC’s policies addressing some forms of misconduct: UC Sexual Violence and Sexual Harassment Policy UC Anti-Discrimination Policy Abusive Conduct in the Workplace Equal Opportunity Employment Statement The University of California is an Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, age, protected veteran status, or other protected status under state or federal law. For the University of California’s Anti-Discrimination Policy, please visit: UC San Diego is a smoke and tobacco free environment. Please visit smokefree.ucsd.edu for more information. UC San Diego Health maintains a marijuana and drug free environment. Employees may be subject to drug screening. #J-18808-Ljbffr UC San Diego Health